Microplate handling systems and related computer program products and methods

• US 9,027,730 B2
• Filed: 09/16/2013
• Issued: 05/12/2015
• Est. Priority Date: 09/16/2008
• Status: Expired due to Fees

• Current Assignee: Ibis Biosciences Incorporated
• Original Assignee: Ibis Biosciences Incorporated
• Inventors: Hofstadler, Steven A., Drader, Jared J., Gutierrez, Jose R., Gleason, Paul J., Bare, Rex O., Miller, Robert D., Smith, Jeffrey C., Oberkramer, Kevin S., Bergold, Ronald K.
• Primary Examiner(s): Singh, Kavel
• Application Number:
  US14/027,781
• Publication Number:
  US 20140154043A1
• Time in Patent Office: 603 Days
• Field of Search: None
• US Class Current: 198/347.1
• CPC Class Codes: G01N 2035/0425 Stacks, magazines or elevators for plates
• CPC Class Codes: G01N 35/0095 introducing urgent samples with priority, e.g. Short Turn Around Time Samples [STATS]
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates

Abstract

Systems, computer program products, and methods useful for handling or managing microplates are provided.

Claims

1. A method of handling a priority microplate in a microplate handling system, the method comprising:

(a) placing the priority microplate in a priority microplate storage unit of the microplate handling system;

(b) transporting a first non-priority microplate from a microplate processing area of the microplate handling system to a non-priority microplate holding area of the microplate handling system using a microplate transport mechanism of the microplate handling system;

(c) placing the first non-priority microplate onto a non-priority microplate holding component disposed in the non-priority microplate holding area using the microplate transport mechanism;

(d) transporting the priority microplate from the priority microplate storage unit to the microplate processing area using the microplate transport mechanism;

(e) transferring material to and/or from one or more selected wells of the priority microplate using a material transfer component of the microplate handling system, and;

(f) transporting the priority microplate from the microplate processing area to a second non-priority microplate storage unit of the microplate handling system or to the priority microplate storage unit using the microplate transport mechanism, thereby handling the priority microplate in the microplate handling system.

2. The method of claim 1, wherein one or more wells of the priority microplate comprise nucleic acid molecules and wherein the method comprises amplifying one or more target regions of the nucleic acid molecules prior to (a).

3. The method of claim 1, comprising transporting the first non-priority microplate from a first non-priority microplate storage unit of the microplate handling system to the microplate processing area using the microplate transport mechanism prior to (b).

4. The method of claim 1, comprising removing material from one or more selected wells of the first non-priority microplate using the material transfer component prior to (b).

5. The method of claim 1, comprising loading a plurality of non-priority microplates in a selected order into the first non-priority microplate storage unit.

6. The method of claim 5, wherein one or more wells of the plurality of non-priority microplates comprise nucleic acids and wherein the method comprises amplifying one or more target regions of the nucleic acids prior to loading the plurality of non-priority microplates into the first non-priority microplate storage unit.

7. The method of claim 1, comprising transporting the first non-priority microplate from the non-priority microplate holding area to the microplate processing area using the microplate transport mechanism.

8. The method of claim 7, comprising transferring material to and/or from one or more selected wells of the first non-priority microplate using the material transfer component.

9. The method of claim 8, comprising transporting the first non-priority microplate from the microplate processing area to the second non-priority microplate storage unit using the microplate transport mechanism.

10. The method of claim 9, comprising transporting a second non-priority microplate from the first non-priority microplate storage unit to the microplate processing area using the microplate transport mechanism.

11. The method of claim 8, wherein the material transfer component comprises one or more needles and wherein the method comprises aspirating one or more aliquots of magnetically responsive particles into the needles from a magnetically responsive particle source prior to or after transferring the material from the selected wells of the first non-priority microplate.

12. The method of claim 11, wherein the material comprises a fluidic material and wherein the method comprises aspirating one or more aliquots of the fluidic material into the needles from the selected wells of the first non-priority microplate.

13. The method of claim 12, comprising transferring the aliquots of magnetically responsive particles and fluidic material to a container of a sample processing station to form a mixture in which the magnetically responsive particles capture at least a first component of the mixture.

14. The method of claim 13, comprising moving and/or retaining the magnetically responsive particles proximal to a surface of the container using a magnetic field and removing at least a second component of the mixture from the container.

15. The method of claim 14, comprising eluting the captured first component from the magnetically responsive particles and detecting a molecular mass of the first component.

16. The method of claim 15, wherein the first component comprises a nucleic acid molecule and wherein the method comprises determining a base composition of the nucleic acid molecule from the molecular mass of the nucleic acid molecule.

17. The method of claim 1, wherein the material transfer component comprises one or more needles and wherein the method comprises aspirating one or more aliquots of magnetically responsive particles into the needles from a magnetically responsive particle source prior to (e).

18. The method of claim 17, wherein the material comprises a fluidic material and wherein (e) comprises aspirating one or more aliquots of the fluidic material into the needles from the selected wells of the priority microplate.

19. The method of claim 18, comprising transferring the aliquots of magnetically responsive particles and fluidic material to a container of a sample processing station to form a mixture in which the magnetically responsive particles capture at least a first component of the mixture.

20. The method of claim 19, comprising moving and/or retaining the magnetically responsive particles proximal to a surface of the container using a magnetic field and removing at least a second component of the mixture from the container.

21. The method of claim 20, comprising eluting the captured first component from the magnetically responsive particles and detecting a molecular mass of the first component.

22. The method of claim 21, wherein the first component comprises a nucleic acid molecule and wherein the method comprises determining a base composition of the nucleic acid molecule from the molecular mass of the nucleic acid molecule.