Recombinant vectors and hosts comprising human glucokinase encoding gene

• US 9,029,142 B2
• Filed: 07/07/2011
• Issued: 05/12/2015
• Est. Priority Date: 01/07/2009
• Status: Active Grant

• Current Assignee: Yiyuan (Shenzhen) Biotech Limtied
• Original Assignee: Haidong Huang
• Inventors: Huang, Haidong
• Primary Examiner(s): Mondesi, Robert
• Assistant Examiner(s): EPSTEIN, TODD MATTHEW
• Application Number:
  US13/178,058
• Publication Number:
  US 20110286984A1
• Time in Patent Office: 1,405 Days
• Field of Search: None
• US Class Current: 435/325
• CPC Class Codes: A01K 2267/0362 Animal model for lipid/glucose metabolism, e.g. obesity, type-2 diabetes
• CPC Class Codes: A61K 31/713 Double-stranded nucleic acids or oligonucleotides
• CPC Class Codes: A61K 38/45 Transferases (2)
• CPC Class Codes: A61K 48/005 characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
• CPC Class Codes: A61P 3/00 Drugs for disorders of the metabolism of the blood or the extracellular fluid A61P7/00
• CPC Class Codes: A61P 3/08 for glucose homeostasis pancreatic hormones A61P5/48
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: C12N 15/86 Viral vectors
• CPC Class Codes: C12N 2710/10343 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 9/1205 Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
• CPC Class Codes: C12Y 207/01002 Glucokinase (2.7.1.2)
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Gene encoding human glucokinase mutant is provided. The gene has the nucleotide sequence chosen from the nucleotide sequence listed as SEQ ID NO:2 and the nucleotide sequence wherein the ORF region encodes the same amino acid sequence as the one encoded by ORF region (position 487 to 1884) of SEQ ID NO:2 and the rest of the region is same as the non-ORF region of SEQ ID NO:2. Human glucokinase mutant encoded by the gene, the recombinant vectors carrying the gene, the hosts comprising the vectors, pharmaceutical compositions thereof, uses thereof, and methods for treating and preventing diseases by using the same are provided. The human glucokinase mutant encoded by the gene has higher activity than that of the wild type human glucokinase, and thus provides a new way of controlling blood glucose and/or preventing and/or treating disturbance of carbohydrate metabolism, especially preventing and treating diabetes.

Claims

1. A human glucokinase encoding gene, wherein the human glucokinase encoding gene is:

an isolated nucleotide sequence as shown by the whole of SEQ ID NO;

2;

or

an isolated nucleotide sequence that is identical to the nucleotide sequence shown by the whole of SEQ ID NO;

2 except the open reading frame (ORF) ranging from position 487 to 1884 of the nucleotide sequence shown by the whole of SEQ ID NO;

2, the ORF encoding the same amino acid sequence as that encoded by the ORF of the nucleotide sequence shown by the whole of SEQ ID NO;

2.

2. A recombinant vector comprising a vector and a gene of interest carried thereon, the gene of interest comprising the human glucokinase encoding gene as set forth in claim 1.

3. The recombinant vector according to claim 2, the vector selected from a group consisting of a cloning vector, a eukaryotic expression vector, a prokaryotic expression vector, and a shuttle vector.

4. The recombinant vector according to claim 2, the vector selected from a group consisting of pIRES2-EGFP, pCMVp-NEO.BAN, pEGFT-Actin, and an adenovirus vector.

5. A host comprising a recombinant vector as set forth in claim 2.

6. The host according to claim 5, the host selected from one or more of Escherichia coli, 293 cells, min-6 cells, and human islet beta-cells.