Method and apparatus for measuring oxidation-reduction potential

• US 9,034,159 B2
• Filed: 12/30/2013
• Issued: 05/19/2015
• Est. Priority Date: 02/28/2011
• Status: Active Grant

• Current Assignee: Caerus Biotechnologies
• Original Assignee: Luoxis Diagnostics, Inc. (Aytu Biopharma, Inc.)
• Inventors: Bar-Or, Raphael, Bar-Or, David, Rael, Leonard T.
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US14/144,154
• Publication Number:
  US 20140110257A1
• Time in Patent Office: 505 Days
• Field of Search: 204416-418, 204/435, 204/400, 204/403.01, 204/403.02
• US Class Current: 204/403.01
• CPC Class Codes: G01N 27/301 Reference electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48
• CPC Class Codes: G01N 27/403 Cells and electrode assemblies
• CPC Class Codes: G01N 27/4168 Oxidation-reduction potential, e.g. for chlorination of water water analysis G01N33/18
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

Methods and systems for measuring the oxidation-reduction potential of a fluid sample are provided. The system includes a test strip with a sample chamber adapted to receive a fluid sample. The sample chamber can be associated with a filter membrane. The test strip also includes a reference cell. The oxidation-reduction potential of a fluid sample placed in the sample chamber can be read by a readout device interconnected to a test lead that is in electrical contact with the sample chamber, and a reference lead that is in electrical contact with the reference cell. Electrical contact between a fluid sample placed in the sample chamber and the reference cell can be established by a bridge. The oxidation-reduction potential may be read as an electrical potential between the test lead and the reference lead of the test strip.

Claims

1. An oxidation-reduction potential test device, comprising:

a substrate;

a sample chamber;

a filter, wherein the filter occupies at least a portion of the sample chamber;

a first test lead supported by the substrate, the first test lead including;

a first area extending into the sample chamber;

a second area extending from the sample chamber;

a second test lead supported by the substrate, the second test lead including;

a first area extending into the sample chamber;

a second area extending from the sample chamber;

a reference cell;

a reference lead, including;

a first area in electrical contact with the reference cell;

a second area extending from the reference cell;

a bridge, wherein the bridge includes at least a portion of the filter, and wherein a fluid sample in the sample chamber is placed in electrical contact with the reference cell by the bridge.

2. The device of claim 1, further comprising:

an overlay, wherein at least a portion of each of the first test lead, the second test lead, the reference cell, the reference lead, and the bridge are held between the substrate and the overlay when the overlay is interconnected to the substrate.

3. The device of claim 2, wherein the overlay includes an aperture, and wherein the aperture corresponds to at least a portion of the sample chamber when the overlay is interconnected to the substrate.

4. The device of claim 2, wherein the first test lead includes a read out portion, wherein the second test lead includes a read out portion, wherein the reference lead includes a readout portion, wherein the read out portion of the first test lead, the read out portion of the second test lead, and the readout portion of the reference lead are accessible to a test device when the overlay is interconnected to the substrate.

5. The device of claim 1, further comprising:

a gel volume, wherein the bridge comprises an electrolytic gel contained within the gel volume, and wherein the electrolytic gel is in contact with the reference cell.

6. The device of claim 5, wherein the gel volume is in communication with the sample chamber.

7. The device of claim 1, wherein the reference cell includes a silver/silver chloride reference cell.

8. The device of claim 1, further comprising:

a wetting agent, wherein the wetting agent is added to at least a bridge portion of the filter, and wherein the bridge and the wetting agent form a salt bridge electrically interconnecting the first area of the first test lead to the reference cell.

9. The device of claim 1, wherein the first test lead is formed from an electrically conductive material having a constant composition.

10. The device of claim 1, further comprising:

a wetting agent, wherein the wetting agent is added to at least a bridge portion of the filter, and wherein the bridge and the wetting agent electrically interconnect the first area of the first test lead to the reference cell.

11. A system for determining the oxidation-reduction potential of a blood product, comprising:

a test strip, the test strip including;

a substrate, wherein a first surface of the substrate defines a first plane;

an overlay, wherein a sample chamber aperture that at least partially defines a sample chamber is formed in the overlay, wherein a first surface of the overlay defines a second plane, and wherein the first and second planes are parallel to one another;

a working electrode, including a sample chamber portion; and

a readout portion, wherein the working electrode is located between the first plane defined by the first surface of the substrate and the second plane defined by the first surface of the overlay;

a counter electrode, including a sample chamber portion; and

a readout portion, wherein the counter electrode is located between the first plane defined by the first surface of the substrate and the second plane defined by the first surface of the overlay;

a reference cell, wherein the reference cell contains a material having a known electrical potential;

a filter;

a bridge component formed at least in part from the filter, wherein the bridge component is in communication with the sample chamber and is electrically interconnected to the reference cell;

a reference electrode, including;

a first portion that is in electrical contact with the reference cell; and

a readout portion;

a blood product, wherein the reference cell is electrically interconnected to the working electrode and the counter electrode by the bridge component when the blood product is placed in the sample chamber;

a voltmeter, wherein the electrical potential between the reference lead and at least one of the working electrode and the counter electrode is read by the voltmeter.

12. The system of claim 11, wherein the working electrode and the counter electrode comprise a material with a constant composition.

13. The system of claim 11, wherein the working electrode and the counter electrode are free of at least one of oxidation-reduction potential and anti-biofouling agents.