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Reagents and methods for PCR

  • US 9,034,605 B2
  • Filed: 03/11/2010
  • Issued: 05/19/2015
  • Est. Priority Date: 03/12/2009
  • Status: Active Grant
First Claim
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1. An amplification reaction mixture comprising:

  • (a) at least one primer pair,(b) a DNA polymerase,(c) dNTP'"'"'s, and(d) at least one double-stranded oligonucleotide additive;

    (i) comprising two separate nucleic acid strands, each of the strands 6-50 nucleotides in length,(ii) that is at least fifty percent double-stranded at 32°

    C.,(iii) having blunt ends or overhangs of 1-8 nucleotides,(iv) that has terminal regions on each of its strands and includes 1-4 modifying groups, each covalently attached to a different terminal region, said modifying groups being polycyclic moieties that do not have bulky portions that are non-planar, and(v) wherein said at least one double-stranded oligonucleotide additive is included in said reaction mixture at a concentration of 25-2000 nM.

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