Systems and methods for yeast propagation

• US 9,034,631 B2
• Filed: 03/14/2013
• Issued: 05/19/2015
• Est. Priority Date: 03/14/2013
• Status: Active Grant

• Current Assignee: Poet Research Inc.
• Original Assignee: Poet Research Inc.
• Inventors: Narendranath, Neelakantam V., Lewis, Stephen M.
• Primary Examiner(s): Weber, Jon P
• Assistant Examiner(s): JOHNSON, KARA D
• Application Number:
  US13/804,364
• Publication Number:
  US 20140273167A1
• Time in Patent Office: 796 Days
• Field of Search: 435/254.21, 435/289.1
• US Class Current: 435/254.21
• CPC Class Codes: C12M 21/18 Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
• CPC Class Codes: C12M 45/09 by enzymatic treatment
• CPC Class Codes: C12N 1/16 Yeasts; Culture media therefor
• CPC Class Codes: C12N 1/18 Baker's yeast; Brewer's yeast
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention relates to systems and methods for propagating yeast using a feedstock material such as starch and one or more enzymes (e.g., an enzyme cocktail) to break down the feedstock material into one or more monosaccharides at a sufficient rate so that the yeast can use the monosaccharides as a carbon source for producing more yeast cells while not producing an undue amount of alcohol.

Claims

1. A method of propagating yeast comprising:

(a) combining

(i) a propagation medium comprising;

a nutrient source;

(ii) a carbon source comprising a starch feedstock material having one or more polysaccharides and/or one or more oligosaccharides;

(iii) one or more amylolytic enzymes that can convert at least a portion of the one or more polysaccharides and/or one or more oligosaccharides into one or more monosaccharides; and

(iv) a first cell mass of yeast, wherein the yeast can use at least a portion of the one or more monosaccharides to grow the first cell mass of the yeast for a time period to form a second cell mass of the yeast; and

(b) enzymatically converting at least a portion of the one or more polysaccharides and/or one or more oligosaccharides into one or more monosaccharides at a temperature in the range from about 25 to 37°

C.; and

(c) growing the first cell mass of yeast on the propagation medium at a temperature in the range from about 25 to 37°

C. for a time period to form the second cell mass of yeast that is greater in cell number than the first cell mass of yeast.

2. The method of claim 1, wherein the one or more enzymes are present in an amount and the one or more polysaccharides and/or one or more oligosaccharides are present in an amount so that substantially no ethanol is produced by the yeast during at least a portion of the time period.

3. The method of claim 2, wherein substantially no ethanol is produced by the yeast during the entire growing time period.

4. The method of claim 1, wherein the one or more enzymes are present in an amount and the one or more polysaccharides and/or one or more oligosaccharides are present in an amount to produce the one or more monosaccharides in an amount to grow the second cell mass of yeast within the time period.

5. The method of claim 4, wherein the one or more monosaccharides comprise glucose and wherein the amount of glucose produced within a 24 hour time period is less than 75 g/L.

6. The method of claim 1, further comprising providing the second cell mass of yeast to a fermentation process.

7. The method of claim 1, wherein the starch material comprises at least one of corn flour, milled corn endosperm, sorghum flour, soybean flour, wheat flour, biomass derived starch, and barley flour.

8. The method of claim 7, wherein the starch material is present in an amount in the range from 15 to 75 grams starch material per liter of the propagation medium.

9. The method of claim 1, wherein the first cell mass of yeast is provided to the propagation medium in an amount in the range from 0.02 to 5 grams dry yeast per liter of the propagation medium.

10. The method of claim 1, wherein the one or more enzymes comprise glucoamylase present in an amount in the range from 0.25 to 30 glucoamylase units (AGUs) per gram of dry solids in the propagation medium.

11. The method of claim 1, wherein the one or more enzymes comprise fungal alpha-amylase present in an amount in the range from 0.03 to 4 fungal alpha-amylase units (FAU-F) per gram of dry solids in the propagation medium.

12. The method of claim 1, wherein the one or more enzymes comprise 0.25 to 30 glucoamylase units (AGUs) per gram of dry solids in the propagation medium and approximately 0.03 to 4 fungal alpha-amylase units (FAU-F) per gram of dry solids in the propagation medium.

13. The method of claim 1, wherein the yeast comprises genetically modified Saccharomyces cerevisiae that can convert at least glucose and/or xylose to ethanol.

14. The method of claim 1, wherein the growing is performed for a time period in the range from 12 to 24 hours.

15. A system for propagating yeast comprising:

(a) a propagation reactor vessel, wherein the propagation vessel contains composition comprising;

(i) a nutrient source;

(ii) a carbon source comprising a starch feedstock material having one or more polysaccharides and/or one or more oligosaccharides;

(iii) one or more amylolytic enzymes that can convert at least a portion of the one or more polysaccharides and/or one or more oligosaccharides into one or more monosaccharides at a temperature in the range from about 25 to 37°

C.; and

(iv) a first cell mass of yeast, wherein the yeast can use at least a portion of the one or more monosaccharides as a feed source;

wherein the propagation reactor vessel is configured for enzymatic hydrolysis of the starch feedstock material, and for growth of the first cell mass of yeast at a temperature in the range from about 25 to 37°

C. to form a second cell mass of the yeast; and

(b) an aerator coupled to the propagation reactor vessel.