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Site-specific serine recombinases and methods of their use

  • US 9,034,650 B2
  • Filed: 02/02/2005
  • Issued: 05/19/2015
  • Est. Priority Date: 02/02/2005
  • Status: Active Grant
First Claim
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1. A method for obtaining site-specific recombination in a eukaryotic cell, the method comprising:

  • providing a eukaryotic cell that comprises a first recombination site and a second recombination site;

    contacting the first and second recombination sites with a prokaryotic serine recombinase polypeptide, resulting in recombination between the recombination sites,wherein the serine recombinase polypeptide can mediate recombination between the first and second recombination sites, the first recombination site is a phage genomic recombination attachment site (attP) or a bacterial genomic recombination attachment site (attB),the second recombination site is attB or attP, andthe serine recombinase is a Streptococcus pyogenes SF370.1 phage serine recombinase,provided that when the first recombination attachment site is attB, the second recombination attachment site is attP, and when the first recombination attachment site is attP, the second recombination attachment site is attB.

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