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Hybrid light chain mice

  • US 9,035,128 B2
  • Filed: 06/22/2011
  • Issued: 05/19/2015
  • Est. Priority Date: 06/22/2010
  • Status: Active Grant
First Claim
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1. A mouse comprising at an endogenous κ

  • light chain locus in its germline;

    (a) an unrearranged light chain variable region comprising a plurality of contiguous unrearranged functional human λ

    light chain V (hVλ

    ) gene segments and a plurality of contiguous unrearranged functional human λ

    light chain J (hJ λ

    ) gene segments, wherein the plurality of hV λ

    gene segments and the plurality of hJ λ

    gene segments are the only functional variable region gene segments in the unrearranged light chain variable region; and

    (b) a mouse κ

    light chain constant region gene,wherein the plurality of contiguous unrearranged functional human λ

    light chain V (hVλ

    ) gene segments and the plurality of contiguous unrearranged functional human λ

    light chain J (hJ λ

    ) gene segments are operably linked to the mouse κ

    light chain constant region gene such that the unrearranged light chain variable region is capable of rearranging to form a rearranged human λ

    light chain variable region and the mouse expresses antibodies comprising a light chain comprising a variable region encoded by the rearranged human λ

    light chain variable region and a constant region encoded by the mouse κ

    light chain constant region gene.

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