Methods and apparatus for detecting molecular interactions using FET arrays
First Claim
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1. A method for sequencing a polypeptide, comprising:
- disposing a plurality of polypeptides into each of a plurality of reaction chambers, at least one reaction chamber of the plurality of reaction chambers coupled to at least one chemical-sensitive field effect transistor (chemFET) having a floating gate;
sequentially introducing amino acyl synthetases into the at least one reaction chamber, the amino acyl synthetase operable to bind to a specific amino acid when present at a free end of the polypeptide;
detecting the presence of an amino acyl synthetase bound to the free end of the polypeptide; and
cleaving the amino acid from the free end of the polypeptide.
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Abstract
Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.
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Citations
20 Claims
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1. A method for sequencing a polypeptide, comprising:
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disposing a plurality of polypeptides into each of a plurality of reaction chambers, at least one reaction chamber of the plurality of reaction chambers coupled to at least one chemical-sensitive field effect transistor (chemFET) having a floating gate; sequentially introducing amino acyl synthetases into the at least one reaction chamber, the amino acyl synthetase operable to bind to a specific amino acid when present at a free end of the polypeptide; detecting the presence of an amino acyl synthetase bound to the free end of the polypeptide; and cleaving the amino acid from the free end of the polypeptide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for sequencing a polypeptide, comprising:
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disposing a plurality of polypeptides into at least one of a plurality of reaction chambers, at least one reaction chamber of the plurality of reaction chambers coupled to at least one chemical-sensitive field effect transistor (chemFET) having a floating gate; sequentially cleaving amino acids from an end of the polypeptide; sequentially detecting the identity of the amino acids by a change in an electrical property of the at least one chemFET having the floating gate. - View Dependent Claims (11, 12, 13, 14, 15, 16)
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17. A method for sequencing a polypeptide, comprising:
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disposing a plurality of polypeptides into at least one of a plurality of reaction chambers, at least one reaction chamber of the plurality of reaction chambers coupled to at least one chemical-sensitive field effect transistor (chemFET) having a floating gate; sequentially cleaving amino acids from an end of the polypeptide; detecting a change in the level of a reaction product of a detection moiety conjugated to an amino acyl synthetase as an indicator of the identity of the amino acid. - View Dependent Claims (18, 19, 20)
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Specification