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Methods and apparatus for detecting molecular interactions using FET arrays

  • US 9,039,888 B2
  • Filed: 09/23/2013
  • Issued: 05/26/2015
  • Est. Priority Date: 12/14/2006
  • Status: Active Grant
First Claim
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1. A method for sequencing a polypeptide, comprising:

  • disposing a plurality of polypeptides into each of a plurality of reaction chambers, at least one reaction chamber of the plurality of reaction chambers coupled to at least one chemical-sensitive field effect transistor (chemFET) having a floating gate;

    sequentially introducing amino acyl synthetases into the at least one reaction chamber, the amino acyl synthetase operable to bind to a specific amino acid when present at a free end of the polypeptide;

    detecting the presence of an amino acyl synthetase bound to the free end of the polypeptide; and

    cleaving the amino acid from the free end of the polypeptide.

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