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Modalities for the treatment of degenerative diseases of the retina

  • US 9,040,038 B2
  • Filed: 04/08/2013
  • Issued: 05/26/2015
  • Est. Priority Date: 01/23/2004
  • Status: Active Grant
First Claim
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1. A method of producing a composition comprising isolated human RPE cells suitable for human therapy, comprising:

  • (a) culturing human pluripotent cells to form clusters of cells;

    (b) culturing the clusters of cells of step (a) under adherent conditions that do not maintain the undifferentiated state of the pluripotent cells for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; and

    (c) isolating and culturing the pigmented cells to form a monolayer comprising cells having a cobblestone, polygonal, epithelial-like appearance and brown pigment dispersed in their cytoplasm, and expressing RPE65 and bestrophin, thereby obtaining human RPE cells.

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