Methods for enriching for pancreatic endocrine cells

• US 9,045,736 B2
• Filed: 12/21/2012
• Issued: 06/02/2015
• Est. Priority Date: 04/21/2008
• Status: Active Grant

• Current Assignee: Viacyte Incorporated (Vertex Pharmaceuticals Incorporated)
• Original Assignee: Viacyte Incorporated (Vertex Pharmaceuticals Incorporated)
• Inventors: Kelly, Olivia, Bang, Anne
• Primary Examiner(s): WILSON, MICHAEL C
• Application Number:
  US13/724,556
• Publication Number:
  US 20130122520A1
• Time in Patent Office: 893 Days
• Field of Search: 435/325
• US Class Current: 1/1
• CPC Class Codes: C12N 2501/50 Cell markers; Cell surface determinants
• CPC Class Codes: C12N 2501/599 with CD designations not provided for elsewhere
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 5/0676 Pancreatic cells
• CPC Class Codes: C12N 5/0678 Stem cells; Progenitor cells; Precursor cells
• CPC Class Codes: G01N 33/56966 Animal cells
• CPC Class Codes: G01N 33/68 involving proteins, peptides or amino acids involving lipoproteins G01N33/92

Abstract

The present disclosure relates to compositions and methods comprising cell surface markers for hES-derived cells, in particular, endoderm lineage cells including pancreatic endoderm-type cells, derived from hES cells.

Claims

1. A method of enriching for cells expressing chromogranin A comprising:

a) exposing an in vitro cell population comprising pancreatic chromogranin A positive cells to a ligand that binds CD200, CD318, CD142, CD57 or CD340; and

b) selecting cells from said in vitro cell population that express CD200, CD318 or CD57 or selecting cells from said in vitro cell population that do not express CD142 or CD340, thereby enriching for cells expressing chromogranin A.

2. The method of claim 1, wherein the ligand is an antibody or fragment thereof.