Linear donor constructs for targeted integration
First Claim
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1. A linear donor nucleic acid molecule comprising homology arms of between 50 and 100 base pairs, a sequence encoding a 2A peptide and a sequence of interest, wherein the homology arms flank the sequence of interest and the sequence encoding the 2A peptide.
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Abstract
Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for integration of an exogenous sequence into a specified region of interest in the genome of a cell.
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23 Claims
- 1. A linear donor nucleic acid molecule comprising homology arms of between 50 and 100 base pairs, a sequence encoding a 2A peptide and a sequence of interest, wherein the homology arms flank the sequence of interest and the sequence encoding the 2A peptide.
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