Biosensor, method of producing the same and detection system comprising the same

• US 9,046,479 B2
• Filed: 06/24/2009
• Issued: 06/02/2015
• Est. Priority Date: 06/24/2008
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Inventors: Takahara, Yoshifumi, Terashima, Noriyoshi, Fujii, Takaaki
• Primary Examiner(s): Ball, J. Christopher
• Assistant Examiner(s): Kessel, Maris R
• Application Number:
  US12/490,699
• Publication Number:
  US 20090321257A1
• Time in Patent Office: 2,169 Days
• Field of Search: 20440301-40315, 205/777.5, 205/792, 205/778, 205/775
• US Class Current: 1/1
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10T 29/4913 Assembling to base an electrical component, e.g., capacitor, etc.

Abstract

A biosensor detects a target substance contained in a liquid sample and includes an insulating base plate having a recess formed in a portion that is thinner than the surrounding part, a working electrode and a counter electrode, at least one of which is disposed in the recess. A reaction reagent is disposed in the recess and reacts with a specific substance in the liquid sample.

Claims

1. A biosensor to detect a target substance contained in a liquid sample, comprising:

an insulating base plate having a first planar side and a second planar side;

a recess formed in a portion of the insulating base plate that is thinner than a surrounding part of the recess, the recess being depressed in a thickness direction from the first planar side to the second planar side;

a convex structure formed in a bottom of the recess and having a peak protruding from the second planar side toward the first planar side;

a working electrode and a counter electrode, at least one of which is disposed along the convex structure; and

a reaction reagent disposed in the recess so as to be in contact with the at least one of the working electrode and the counter electrode, the reaction reagent reacting with a specific substance in the liquid sample;

a spacer disposed on the first planar side of the insulating base plate so that at least part of the recess is exposed;

an upper cover disposed over the spacer to cover the recess exposed from the spacer;

a liquid sample chamber formed by the insulating base plate including the recess, the spacer, and the upper cover; and

an intake port disposed above the recess and configured to draw a liquid sample by capillary action from the outside into the liquid sample chamber,

wherein the liquid sample chamber includes a first space and a second space disposed on the first space so as to communicate with the first space,

the first space is formed by the recess, and

the second space is formed by the intake port.

2. The biosensor according to claim 1,

wherein the recess further includes a concave structure depressed in the thickness direction extending between the first planar side and the second planar side of the insulating base plate.

3. The biosensor according to claim 2,

wherein the concave structure includes a valley shape that narrows in width toward the second planar side of the insulating base plate.

4. The biosensor according to claim 2,

wherein the convex structure includes a peak shape that tapers toward a distal end of the peak.

5. The biosensor according to claim 2,

wherein the shape of the concave structure or the convex structure in a planar direction of the base plate is a dot shape, a linear shape, a square shape, or a curved shape, or is a combination of two or more of these.

6. The biosensor according to claim 2,

wherein an outer edge of the recess is circular, and

the concave structure and the convex structure are provided alternately on a bottom face of the recess to scribe a circular shape concentric with the outer edge of the recess.

7. The biosensor according to claim 2,

wherein an outer edge of the recess is circular, and

the concave structure is provided in a grid pattern on a bottom face of the recess, and portions surrounded by the concave structure are formed with the convex structures.

8. The biosensor according to claim 2,

wherein an outer edge of the recess is rectangular, and

the concave structure is provided in a grid pattern on a bottom face of the recess, and portions surrounded by the concave structure are formed with the convex structures.

9. The biosensor according to claim 1,

further comprising a liquid sample chamber arranged to hold the liquid sample, above the recess in the thickness direction of the base plate.

10. The biosensor according to claim 1,

further comprising a vent port arranged to discharge air from an inside of the liquid sample chamber to the outside.

11. The biosensor according to claim 1,

wherein at least part of a side face of the recess is not perpendicular to the first and second planar sides of the base plate.

12. The biosensor according to claim 1,

further comprising terminals arranged to electrically connect the working electrode and the counter electrode with a detection device to detect a target substance from a current value of the biosensor.

13. The biosensor according to claim 1,

wherein the reaction reagent is disposed on the working electrode and the counter electrode.

14. The biosensor according to claim 1,

wherein the working electrode or the counter electrode contains an electroconductive substance.

15. The biosensor according to claim 14,

wherein the electroconductive substance includes an inorganic electroconductive substance.

16. The biosensor according to claim 15,

wherein the working electrode or the counter electrode contains palladium.

17. The biosensor according to claim 14,

wherein the electroconductive substance includes an organic electroconductive substance.

18. The biosensor according to claim 17,

wherein the working electrode or the counter electrode contains an electroconductive polymer.

19. The biosensor according to claim 1,

wherein an enzyme is disposed in the recess.

20. The biosensor according to claim 19,

wherein oxidase or dehydrogenase is disposed in the recess.

21. The biosensor according to claim 20,

wherein oxidase or dehydrogenase is disposed in the recess, and

a substrate of the oxidase and the dehydrogenase is glucose.

22. The biosensor according to claim 1,

wherein an electron transfer substance is disposed in the recess.

23. The biosensor according to claim 22,

wherein hexacyano iron (III) potassium is disposed in the recess.

24. The biosensor according to claim 1,

wherein the reaction reagent is in a dry state.

25. The biosensor according to claim 1,

wherein the working electrode and the counter electrode are configured to measure a hemocyte component in a liquid sample.

26. The biosensor according to claim 1,

wherein the working electrode and the counter electrode are configured to measure readily oxidizable substances in a liquid sample.

27. The biosensor according to claim 1,

wherein the electrodes are configured to detect intake of a liquid sample.

28. The biosensor according to claim 1, comprising:

the insulating base plate as a first plate; and

a second plate that is insulating and disposed opposite the first plate,

wherein the reaction reagent and one electrode from among the working electrode and the counter electrode are disposed in the recess on the first plate, and

the other electrode from among the working electrode and the counter electrode is disposed on the second plate.