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Molecular redundant sequencing

  • US 9,051,611 B2
  • Filed: 08/14/2013
  • Issued: 06/09/2015
  • Est. Priority Date: 07/26/2007
  • Status: Active Grant
First Claim
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1. A method of sequencing nucleic acid fragments within a genetic target material, comprising:

  • fractionating the genetic target material into separate fractions;

    tagging each of said separate fractions with known exogenous registration sequences, wherein nucleic acid fragments in a single fraction are tagged with an identical known exogenous registration sequence and wherein nucleic acid fragments in said separate fractions are tagged with different known exogenous registration sequences; and

    further wherein the exogenous registration sequences provide identification of which separate fraction a given nucleic acid fragment was derived from, thereby generating tagged fragments in each of said separate fractions;

    circularizing the tagged fragments;

    pooling the tagged fragments from all of said separate fractions;

    sequencing the tagged fragments until the exogenous registration sequence has been sequenced at least twice, thereby generating sequence reads, each of which comprises at least two exogenous registration sequence portions and at least one tagged fragment sequence portion.

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