Methods and apparatus for centrifugal liquid chromatography
First Claim
1. A centrifugal chromatographic system comprising:
- a chromatographic enclosure;
a rotor that carries the chromatographic enclosure wherein the rotor is configured to rotate the chromatographic enclosure;
a fluid delivery mechanism in fluid communication with the chromatographic enclosure including one or more flow conduits configured to facilitate the delivery of a mobile phase fluid to the chromatographic enclosure on the rotor while the rotor is rotating and the fluid delivery mechanism includes a first portion configured to remain stationary while the rotor is rotating and a second portion carried on the rotor and configure to rotate with the rotor arranged so that fluid can be passed from the first portion to the second portion of the fluid delivery mechanism while the rotor is rotating such that approximately steady fluid flow conditions are established on the rotor;
a sample introduction mechanism in fluid communication with the chromatographic enclosure, said sample introduction mechanism being arranged to introduce a sample fluid to the chromatographic enclosure while the rotor is rotating, wherein the sample introduction mechanism is configured to receive a sample introduction signal and to trigger the introduction of the sample fluid in response to receiving the sample introduction signal.
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Abstract
Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.
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Citations
26 Claims
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1. A centrifugal chromatographic system comprising:
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a chromatographic enclosure; a rotor that carries the chromatographic enclosure wherein the rotor is configured to rotate the chromatographic enclosure; a fluid delivery mechanism in fluid communication with the chromatographic enclosure including one or more flow conduits configured to facilitate the delivery of a mobile phase fluid to the chromatographic enclosure on the rotor while the rotor is rotating and the fluid delivery mechanism includes a first portion configured to remain stationary while the rotor is rotating and a second portion carried on the rotor and configure to rotate with the rotor arranged so that fluid can be passed from the first portion to the second portion of the fluid delivery mechanism while the rotor is rotating such that approximately steady fluid flow conditions are established on the rotor; a sample introduction mechanism in fluid communication with the chromatographic enclosure, said sample introduction mechanism being arranged to introduce a sample fluid to the chromatographic enclosure while the rotor is rotating, wherein the sample introduction mechanism is configured to receive a sample introduction signal and to trigger the introduction of the sample fluid in response to receiving the sample introduction signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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25. A centrifugal chromatographic system comprising:
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a chromatographic enclosure; a rotor that carries the chromatographic enclosure wherein the rotor is configured to rotate the chromatographic enclosure; a fluid delivery mechanism in fluid communication with the chromatographic enclosure including one or more flow conduits configured to facilitate the delivery of a mobile phase fluid to the chromatographic enclosure on the rotor while the rotor is rotating and the fluid delivery mechanism includes a first portion configured to remain stationary while the rotor is rotating and a second portion carried on the rotor and configure to rotate with the rotor arranged so that fluid can be passed from the first portion to the second portion of the fluid delivery mechanism while the rotor is rotating such that approximately steady fluid flow conditions are established on the rotor; a sample introduction mechanism in fluid communication with the chromatographic enclosure the sample introduction mechanism being arranged to introduce a sample fluid to the chromatographic enclosure while the rotor is rotating; and a controller, coupled to the sample introduction mechanism and arranged to automatically trigger the introduction of the sample fluid. - View Dependent Claims (26)
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Specification