Device for cell culture

• US 9,057,047 B2
• Filed: 08/06/2009
• Issued: 06/16/2015
• Est. Priority Date: 08/06/2008
• Status: Active Grant

• Current Assignee: RigeneranD SRL (Evotec SE)
• Original Assignee: RigeneranD SRL (Evotec SE)
• Inventors: Conte, Pierfranco, Dominici, Massimo, Bellini, Gianni, Brogli, Matteo
• Primary Examiner(s): HOBBS, MICHAEL L
• Application Number:
  US13/056,111
• Publication Number:
  US 20110129913A1
• Time in Patent Office: 2,140 Days
• Field of Search: 435/284.1, 435/289.1, 435/293.1, 435/297.5, 435/299.1
• US Class Current: 1/1
• CPC Class Codes: C12M 23/00 Constructional details, e.g. recesses, hinges flow directing inserts in C12M27/18-C12M27/24; apparatus for chemical or physical processes in general B01J, chemical or physical laboratory apparatus in general B01L
• CPC Class Codes: C12M 23/14 Bags
• CPC Class Codes: C12M 23/24 Gas permeable parts
• CPC Class Codes: C12M 23/34 Internal compartments or partitions
• CPC Class Codes: C12M 29/10 Perfusion
• CPC Class Codes: C12M 37/00 Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination C12M23/38 takes precedence; filtration in general and filters per se B01D24/00-B01D41/00; autoclaves B01J3/04; treatment of microorganisms with electrical or wave energy C12N13/00

Abstract

A device for cell culture includes a container body, which has an internal compartment to contain a quantity of cells to be cultured and a culture surface for the cells placed in the internal compartment; an introduction opening for introducing the cells to be cultured in the internal compartment; the container body having a first half shell and a second half shell, which can be fixed in mutually facing relation by a removable fixing system and which together define the internal compartment and the culture surface; and at least one out feed opening for a transport and/or culture fluid for the cells.

Claims

1. A device for cell culture, comprising:

a container body which has an internal compartment to contain a quantity of cells to be cultured and a culture surface for said cells placed in said internal compartment; and

an introduction opening for introducing said cells to be cultured in said compartment;

wherein said container body comprises;

a first semi-shell and a second semi-shell configured to be fixed facing each other by a removable fixing system and together to define said internal compartment and said culture surface; and

at least one outfeed opening of at least one of a transport or a culture fluid of said cells,

wherein said first semi-shell and second semi-shell comprise respective flexible laminar sheets, at least one of which is made from a gas-permeable, waterproof and microbiologically impermeable material,

wherein said culture surface that comprises an internal surface of at least one of said first semi-shell and second semi-shell comprises a reticular element comprising threads arranged in a warp and a weft, and

wherein said culture surface further comprises a porous or rough three-dimensional surface of a culture element disposed in said internal compartment by adhering to at least one of said first semi-shell or second semi-shell.

2. The device according to claim 1, wherein said culture element is made from a bio-absorbable material.

3. The device according to claim 1, wherein said culture element is made from a degradable material according to pre-set degradation times.

4. The device according to claim 1, wherein said threads of said warp comprise segments of capillaries that are parallel and equal to each other, defining respective opposite homologous ends, and wherein said weft comprises at least two connection elements, each configured to connect homologous ends of said opposite homologous ends.

5. The device according to claim 4, wherein said connection elements respectively comprise an infeed and an outfeed for an auxiliary fluid arranged to flow through said segments of capillaries and through said connection elements.

6. The device according to claim 1, wherein said fixing system comprises a peelable gluing bead.

7. The device according to claim 1, wherein said fixing system comprises:

a frame element) that delimits said internal compartment and which defines two opposite faces; and

respective peelable gluing cords applied on each face of said opposite faces, in perimeter zones of said internal compartment, said frame element being configured to receive in removable gluing said first shell and said second shall, each one on a respective face of said opposite faces.

8. The device according to claim 1, wherein one or more of said culture surface or said culture element are configured to be directly applied to a patient.

9. The device according to claim 1, wherein said culture surface or culture element are made from materials that are degradable in a pre-set time interval.

10. The device according to claim 1, wherein said culture surface comprises a size increase system to increase a size of said culture surface.

11. The device according to claim 10, wherein said size increase system comprises a series of bends obtained in said culture surface and in relief in said internal compartment.

12. An apparatus for cell culture, comprising:

a device for cell culture having a container body, the container body comprising,

an internal compartment configured to contain a quantity of cells to be cultured,

a culture surface for said cells placed in said internal compartment,

a first semi-shell and a second semi-shell configured to be fixed facing each other by a removable fixing system and together to define said internal compartment and said culture surface,

at least one outfeed opening of at least one of a transport or a culture fluid of said cells; and

an introduction opening for introducing said cells to be cultured in said compartment,

wherein said first semi-shell and second semi-shell comprise respective flexible laminar sheets, at least one of which is made from a gas-permeable, waterproof and microbiologically impermeable material,

wherein said culture surface that comprises an internal surface of at least one of said first semi-shell and second semi-shell comprises a reticular element that includes thread arranged in a warp and a weft, and

wherein said culture surface further comprises a porous or rough three-dimensional surface of a culture element disposed in said internal compartment by adhering to at least one of said first semi-shell or second semi-shell.