Arrays and methods of use
First Claim
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1. A method of producing a molecular array comprising:
- (i) preselecting a plurality of oligonucleotides to be immobilised;
(ii) immobilising at least a portion of the plurality of oligonucleotides on a solid support at a density to allow each of said at least a portion of the plurality of oligonucleotides on the solid support to be individually resolved upon labeling, thereby forming two or more separate and discrete elements, at least two elements of said two or more elements being spatially addressable, each of said at least two elements comprising a plurality of immobilized oligonucleotides from said at least a portion of the plurality of oligonucleotides,wherein sequence identities of said at least a portion of the plurality of immobilised oligonucleotides in each of said at least two elements are specified by a location of each of said at least two elements in which the oligonucleotides are contained;
(iii) labeling at least a portion of the plurality of immobilised oligonucleotides at each of said at least two elements with one or more labels, thereby producing labeled immobilised oligonucleotides; and
(iv) analysing whether at least a portion of the labeled immobilised oligonucleotides of said at least two elements are individually optically resolvable from another portion of the labeled immobilised oligonucleotides, whereby said at least a portion of the labeled immobilized oligonucleotides on each of said at least two elements are individually optically resolvable from the another portion of the labeled immobilised oligonucleotides.
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Abstract
Methods are provided for producing a molecular array comprising a plurality of molecules immoblised to a solid substrate at a density which allows individual immobilised molecules to be individually resolved, wherein each individual molecule in the array is spatially addressable and the identity of each molecule is known or determined prior to immobilisation. The use of spatially addressable low density molecular arrays in single molecule detection and analysis techniques is also provided. Novel assays and methods are also provided.
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Citations
27 Claims
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1. A method of producing a molecular array comprising:
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(i) preselecting a plurality of oligonucleotides to be immobilised; (ii) immobilising at least a portion of the plurality of oligonucleotides on a solid support at a density to allow each of said at least a portion of the plurality of oligonucleotides on the solid support to be individually resolved upon labeling, thereby forming two or more separate and discrete elements, at least two elements of said two or more elements being spatially addressable, each of said at least two elements comprising a plurality of immobilized oligonucleotides from said at least a portion of the plurality of oligonucleotides, wherein sequence identities of said at least a portion of the plurality of immobilised oligonucleotides in each of said at least two elements are specified by a location of each of said at least two elements in which the oligonucleotides are contained; (iii) labeling at least a portion of the plurality of immobilised oligonucleotides at each of said at least two elements with one or more labels, thereby producing labeled immobilised oligonucleotides; and (iv) analysing whether at least a portion of the labeled immobilised oligonucleotides of said at least two elements are individually optically resolvable from another portion of the labeled immobilised oligonucleotides, whereby said at least a portion of the labeled immobilized oligonucleotides on each of said at least two elements are individually optically resolvable from the another portion of the labeled immobilised oligonucleotides. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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Specification