Microfluidic systems and methods for thermal control

• US 9,061,278 B2
• Filed: 06/29/2010
• Issued: 06/23/2015
• Est. Priority Date: 06/29/2009
• Status: Active Grant

• Current Assignee: Canon USA, Inc. (Canon Inc.)
• Original Assignee: Canon USA, Inc. (Canon Inc.)
• Inventors: Hasson, Kenton C., Coursey, Johnathan S., Owen, Gregory H., Inoue, Hiroshi
• Primary Examiner(s): Bullock, In Suk
• Assistant Examiner(s): EOM, ROBERT J
• Application Number:
  US12/825,476
• Publication Number:
  US 20110048547A1
• Time in Patent Office: 1,820 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/14 Process control and prevention of errors
• CPC Class Codes: B01L 2200/147 Employing temperature sensors
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/1805 Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/525 with physical movement of samples between temperature zones
• CPC Class Codes: C12Q 3/00 Condition responsive control processes apparatus therefor C12M1/36; controlling or regulating in general G05
• CPC Class Codes: H05B 1/02 Automatic switching arrangements specially adapted to apparatus ; Control of heating devices thermally-actuated switches H01H37/00
• CPC Class Codes: Y10T 137/6606 With electric heating element

Abstract

The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed resistive thermal detectors (RTDs). Each of the RTDs is associated with one of the microfluidic channels. The RTDs are connected to a power supply through individual electrodes and pairs of common electrodes. Adjacent RTDs may be driven with alternating polarities, and the current in the common electrodes may be minimized using a virtual ground circuit. The compact microfluidic device is capable of fast heating and highly precise thermal control. The compact microfluidic device is also capable using the RTDs to sense temperature without their heating capability.

Claims

1. A method for individually controlling a plurality of resistive thermal detectors (RTDs) of a microfluidic device of a microfluidic system, wherein the RTDs are each adjacent to a portion of an associated one of the plurality of microchannels, the method comprising:

generating heater control signals having alternating polarities to drive the plurality of RTDs;

supplying the heater control signals to the plurality of RTDs so that adjacent RTDs of the plurality of RTDs are driven with heater control signals having opposite polarities relative to each other;

minimizing current in first and second common electrodes by connecting the first and second common electrodes to a virtual ground circuit, wherein the first and second common electrodes are each connected to each RTD of the plurality of RTDs;

sensing a temperature of each of the plurality of RTDs by using the first and second common electrodes; and

updating the heater control signals using the sensed temperatures of the plurality of RTDs.

2. The method of claim 1, wherein the heater control signals are generated and updated based on a polymerase chain reaction (PCR) profile or on a temperature ramp profile.

3. The method of claim 1, wherein the minimizing the current in the first and second common electrodes comprises determining a current imbalance between currents of the heating control signals supplied to the plurality of RTDs.

4. The method of claim 3, wherein the minimizing the current in the first and second common electrodes further comprises sourcing/sinking the determined current imbalance.

5. The method of claim 1, further comprising preventing the heater control signals from having a voltage lower than a minimum voltage limit.

6. The method of claim 1, wherein the heater control signals are generated so that deoxyribonucleic acid (DNA) contained in the portions of the associated ones of the plurality of microchannels is amplified through a polymerase chain reaction (PCR).

7. The method of claim 1, wherein the heater control signals are generated so as to ramp the temperature of the first and second RTDs.

8. The method of claim 1, wherein the microfluidic device further comprises a second plurality of RTDs;

the method further comprising;

generating second heater control signals having alternating polarities to drive the second plurality of RTDs;

supplying the second heater control signals to the second plurality of RTDs so that adjacent RTDs of the second plurality of RTDs are driven with second heater control signals having opposite polarities;

minimizing current in third and fourth common electrodes, wherein the third and fourth common electrodes are each connected to each RTD of the second plurality of RTDs;

sensing a temperature of each of the second plurality of RTDs; and

updating the second heater control signals using the sensed temperatures of the second plurality of RTDs.

9. The method of claim 8, wherein each of the second plurality of RTDs is adjacent to a second portion of an associated one of the plurality of microchannels;

the heater control signals that drive the plurality of RTDs are generated so that deoxyribonucleic acid (DNA) contained in the associated ones of the plurality of microchannels is amplified;

the second heater control signals that drive the second plurality of RTDs are generated so as to ramp the temperature of the second plurality of RTDs.

10. The method of claim 9, wherein the DNA amplification is achieved through a polymerase chain reaction (PCR).

11. The method of claim 8, wherein the microfluidic device further comprises a second plurality of microchannels, each of the second plurality of RTDs being adjacent to a portion of an associated one of the second plurality of microchannels;

and

the first and second heater control signals that respectively drive the first and second plurality of RTDs are generated so that deoxyribonucleic acid (DNA) contained in the portions of the associated ones of the plurality of microchannels and the second plurality of microchannels is amplified.

12. The method of claim 11, wherein the DNA amplification is achieved through a polymerase chain reaction (PCR).

13. The method of claim 8, wherein the microfluidic device further comprises a second plurality of microchannels, each of the second plurality of RTDs being adjacent to a portion of an associated one of the second plurality of microchannels;

and

the first and second heater control signals that respectively drive the first and second plurality of RTDs are generated to ramp the temperature of the first and second plurality of RTDs.

14. The method of claim 1, wherein the heater control signals are updated by modulating the amplitude of the heater control signals.

15. The method of claim 1, wherein the heater control signals are alternating current signals.

16. The method of claim 15, wherein the heater control signals have opposite polarities when they are 180 degrees out of phase with each other.