Dual specific binding proteins directed against IL-1 and/or IL-17
First Claim
Patent Images
1. A method of treating a subject for an inflammatory disorder, comprising administering a binding protein to the subject, wherein the binding protein comprises first and second polypeptide chains, each independently comprising VD1-(X1)n-VD2-C-X2, whereinVD1 is a first variable domain;
- VD2 is a second variable domain;
C is a constant domain;
X1 is a linker;
X2 is an Fc region that is either present or absent; and
n is 0 or 1;
wherein the VD1 domains on the first and second polypeptide chains form a first functional target binding site and the VD2 domains on the first and second polypeptide chains form a second functional target binding site, and wherein the binding protein is capable of binding IL-1β and
IL-17, wherein(i) the variable domains that form a functional target binding site for IL-1β
comprise;
CDRs 1-3 from SEQ ID NO;
32 and CDRs 1-3 from SEQ ID NO;
33,CDRs 1-3 from SEQ ID NO;
34 and CDRs 1-3 from SEQ ID NO;
35,CDRs 1-3 from SEQ ID NO;
36 and CDRs 1-3 from SEQ ID NO;
37,CDRs 1-3 from SEQ ID NO;
38 and CDRs 1-3 from SEQ ID NO;
39, orCDRs 1-3 from SEQ ID NO;
40 and CDRs 1-3 from SEQ ID NO;
41;
and(ii) the variable domains that form a functional target binding site for IL-17 compriseCDRs 1-3 from SEQ ID NO;
44 and CDRs 1-3 from SEQ ID NO;
45.
1 Assignment
0 Petitions
Accused Products
Abstract
Engineered multivalent and multispecific binding proteins that bind IL-1β and/or IL-17 are provided, along with methods of making and uses in the prevention, diagnosis, and/or treatment of disease.
272 Citations
34 Claims
-
1. A method of treating a subject for an inflammatory disorder, comprising administering a binding protein to the subject, wherein the binding protein comprises first and second polypeptide chains, each independently comprising VD1-(X1)n-VD2-C-X2, wherein
VD1 is a first variable domain; -
VD2 is a second variable domain; C is a constant domain; X1 is a linker; X2 is an Fc region that is either present or absent; and n is 0 or 1; wherein the VD1 domains on the first and second polypeptide chains form a first functional target binding site and the VD2 domains on the first and second polypeptide chains form a second functional target binding site, and wherein the binding protein is capable of binding IL-1β and
IL-17, wherein(i) the variable domains that form a functional target binding site for IL-1β
comprise;CDRs 1-3 from SEQ ID NO;
32 and CDRs 1-3 from SEQ ID NO;
33,CDRs 1-3 from SEQ ID NO;
34 and CDRs 1-3 from SEQ ID NO;
35,CDRs 1-3 from SEQ ID NO;
36 and CDRs 1-3 from SEQ ID NO;
37,CDRs 1-3 from SEQ ID NO;
38 and CDRs 1-3 from SEQ ID NO;
39, orCDRs 1-3 from SEQ ID NO;
40 and CDRs 1-3 from SEQ ID NO;
41;and (ii) the variable domains that form a functional target binding site for IL-17 comprise CDRs 1-3 from SEQ ID NO;
44 and CDRs 1-3 from SEQ ID NO;
45.- View Dependent Claims (2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 34)
VD2 is a second heavy chain variable domain; C is a heavy chain constant domain; X1 is a linker; X2 is an Fc region that is either present or absent; n is 0 or 1, and a second polypeptide chain comprising VD1-(X1)n-VD2-C, wherein VD1 is a first light chain variable domain; VD2 is a second light chain variable domain; C is a light chain constant domain; X1 is a linker; n is 0 or 1, wherein the VD1 domains on the first and second polypeptide chains form a first functional target binding site and the VD2 domains on the first and second polypeptide chains form a second functional target binding site.
-
-
5. The method of claim 1, wherein
(i) the binding protein is capable of binding IL-1β - with a KD of about 5.1×
10−
11 M, as measured by surface plasmon resonance, or capable of inhibiting IL-1β
with an IC50 of about 2.563 nM, as measured in an IL-1β
neutralization assay, and/or(ii) the binding protein is capable of binding IL-17 with a KD of about 4.8×
10−
12 M, as measured by surface plasmon resonance, or capable of inhibiting IL-17 with an IC50 of about 1.7 nM, as measured in an IL-17 neutralization assay.
- with a KD of about 5.1×
-
6. The method of claim 1, wherein the binding protein comprises
(i) variable domains that form a functional target binding site for IL-1β - comprising;
(1) SEQ ID NO;
32 and SEQ ID NO;
33,(2) SEQ ID NO;
34 and SEQ ID NO;
35,(3) SEQ ID NO;
36 and SEQ ID NO;
37,(4) SEQ ID NO;
38 and SEQ ID NO;
39, or(5) SEQ ID NO;
40 and SEQ ID NO;
41;and (ii) variable domains that form a functional target binding site for IL-17 comprising; SEQ ID NO;
44 and SEQ ID NO;
45.
- comprising;
-
7. The method of claim 1, wherein the binding protein comprises two first polypeptide chains and two second polypeptide chains and four functional target binding sites.
-
8. The method of claim 1, wherein X1 is any one of SEQ ID NO:
- 1-31.
-
9. The method of claim 1, wherein the Fc region of the binding protein is a variant sequence Fc region.
-
10. The method of claim 1, wherein the Fc region of the binding protein is an Fc region from an IgG1, IgG2, IgG3, IgG4, IgA, IgM, IgE, or IgD.
-
11. The method of claim 1, wherein the binding protein comprises
(a) a heavy chain constant region comprising a wild type human IgG1 heavy chain sequence; - and
(b) a light chain constant region comprising a wild type human kappa light chain constant region sequence.
- and
-
12. The method of claim 1, wherein the binding protein comprises
(a) a heavy chain constant region comprising a human IgG1 heavy chain sequence modified by one or more amino acid changes, wherein the changes comprise substitution of leucines at positions 234 and 235 with alanines, wherein the amino acid positions are numbered using EU index numbering; - and
(b) a light chain constant region comprising a wild type human kappa light chain constant region sequence.
- and
-
13. The method of claim 1, wherein the binding protein is a crystallized binding protein.
-
14. The method of claim 1, wherein the binding protein comprises
(i) variable domains that form a functional target binding site for IL-1β - comprising CDRs 1-3 from SEQ ID NO;
32 and CDRs 1-3 from SEQ ID NO;
33, and(ii) variable domains that form a functional target binding site for IL-17 comprising CDRs 1-3 from SEQ ID NO;
44 and CDRs 1-3 from SEQ ID NO;
45.
- comprising CDRs 1-3 from SEQ ID NO;
-
15. The method of claim 1, wherein the binding protein comprises
(i) variable domains that form a functional target binding site for IL-1β - comprising SEQ ID NO;
32 and SEQ ID NO;
33, and(ii) variable domains that form a functional target binding site for IL-17 comprising SEQ ID NO;
44 and SEQ ID NO;
45.
- comprising SEQ ID NO;
-
16. The method of claim 1, wherein the first polypeptide chain of the binding protein comprises SEQ ID NO:
- 98 and the second polypeptide chain of the binding protein comprises SEQ ID NO;
99.
- 98 and the second polypeptide chain of the binding protein comprises SEQ ID NO;
-
17. The method of claim 16, wherein the binding protein comprises
(a) a heavy chain constant region on the first polypeptide chain comprising a human IgG1 heavy chain sequence modified by one or more amino acid changes, wherein the changes comprise substitution of leucines at positions 234 and 235 with alanines, wherein the amino acid positions are numbered using EU index numbering; - and
(b) a light chain constant region on the second polypeptide chain comprising a human kappa light chain constant region sequence.
- and
-
18. The method of claim 16, wherein:
-
(i) the binding protein is capable of binding IL-1β
with a KD of about 5.1×
10−
11 M, as measured by surface plasmon resonance, or capable of inhibiting IL-1β
with an IC50 of about 0.027 nM, as measured in an IL-1β
neutralization assay, and/or(ii) the binding protein is capable of binding IL-17 with a KD of about 4.8×
10−
12 M, as measured by surface plasmon resonance, or capable of inhibiting IL-17 with an IC50 of about 0.091 nM, as measured in an IL-17 neutralization assay.
-
-
19. The method of claim 1, wherein the binding protein comprises
(i) variable domains that form a functional target binding site for IL-1β - comprising CDRs 1-3 from SEQ ID NO;
34 and CDRs 1-3 from SEQ ID NO;
35, and(ii) variable domains that form a functional target binding site for IL-17 comprising CDRs 1-3 from SEQ ID NO;
44 and CDRs 1-3 from SEQ ID NO;
45.
- comprising CDRs 1-3 from SEQ ID NO;
-
20. The method of claim 1, wherein the binding protein comprises
(i) variable domains that form a functional target binding site for IL-1β - comprising SEQ ID NO;
34 and SEQ ID NO;
35, and(ii) variable domains that form a functional target binding site for IL-17 comprising SEQ ID NO;
44 and SEQ ID NO;
45.
- comprising SEQ ID NO;
-
21. The method of claim 19, wherein X1 on the first polypeptide chain comprises SEQ ID NO:
- 29 and X1 on the second polypeptide chain comprises SEQ ID NO;
30.
- 29 and X1 on the second polypeptide chain comprises SEQ ID NO;
-
22. The method of claim 2, wherein the binding protein comprises
(a) a heavy chain constant region on the first polypeptide chain comprising a human IgG1 heavy chain sequence modified by one or more amino acid changes, wherein the changes comprise substitution of leucines at positions 234 and 235 with alanines, wherein the amino acid positions are numbered using EU index numbering; - and
(b) a light chain constant region on the second polypeptide chain comprising a human kappa light chain constant region sequence.
- and
-
23. The method of claim 2, wherein:
-
(i) the binding protein is capable of binding IL-1β
with a KD of about 3.4×
10−
11 M, as measured by surface plasmon resonance, or capable of inhibiting IL-1β
with an IC50 of about 0.018 nM, as measured in an IL-1β
neutralization assay, and/or(ii) the binding protein is capable of binding IL-17 with a KD of about 4.8×
10−
12 M, as measured by surface plasmon resonance, or capable of inhibiting IL-17 with an IC50 of about 0.068 nM, as measured in an IL-17 neutralization assay.
-
-
24. The method of claim 1, wherein the binding protein comprises any one of:
-
DVD2423 (comprising SEQ ID NOs;
48 and
49);DVD2424 (comprising SEQ ID NOs;
50 and
51);DVD2425 (comprising SEQ ID NOs;
52 and
53);DVD2426 (comprising SEQ ID NOs;
54 and
55);DVD2427 (comprising SEQ ID NOs;
56 and
57);DVD2428 (comprising SEQ ID NOs;
58 and
59);DVD2429 (comprising SEQ ID NOs;
60 and
61);DVD2430 (comprising SEQ ID NOs;
62 and
63);DVD2431 (comprising SEQ ID NOs;
64 and
65);DVD2432 (comprising SEQ ID NOs;
66 and
67);DVD2433 (comprising SEQ ID NOs;
68 and
69);DVD2434 (comprising SEQ ID NOs;
70 and
71);DVD2435 (comprising SEQ ID NOs;
72 and
73);DVD2436 (comprising SEQ ID NOs;
74 and
75);DVD2437 (comprising SEQ ID NOs;
76 and
77);DVD2438 (comprising SEQ ID NOs;
78 and
79);DVD2439 (comprising SEQ ID NOs;
80 and
81);DVD2440 (comprising SEQ ID NOs;
82 and
83);DVD2441 (comprising SEQ ID NOs;
84 and
85);DVD2442 (comprising SEQ ID NOs;
86 and
87);DVD3415 (comprising SEQ ID NOs;
98 and
99); andDVD3418 (comprising SEQ ID NOs;
104 and
105).
-
-
25. The method of claim 1, further comprising administering a second agent.
-
34. The method of claim 2, wherein the disorder is keratoconjunctivitis sicca.
-
3. A method of treating a subject for a disorder selected from the group consisting of asthma, rheumatoid arthritis, juvenile idiopathic arthritis, psoriatic arthritis, psoriasis, plaque psoriasis, keratoconjunctivitis sicca, blepharitis, keratitis, uveitis, Crohn'"'"'s disease, ulcerative colitis, inflammatory bowel disease (IBD), insulin dependent diabetes mellitus, ankylosing spondylitis, spondyloarthropathy, chronic obstructive pulmonary disease (COPD), cystic fibrosis, urticaria, ectopic eczema, osteoarthritis, and multiple sclerosis, the method comprising administering a binding protein to the subject, wherein the binding protein comprises first and second polypeptide chains, each independently comprising VD1-(X1)n-VD2-C-X2, wherein
VD1 is a first variable domain; -
VD2 is a second variable domain; C is a constant domain; X1 is a linker; X2 is an Fc region that is either present or absent; and n is 0 or 1; wherein the VD1 domains on the first and second polypeptide chains form a first functional target binding site and the VD2 domains on the first and second polypeptide chains form a second functional target binding site, and wherein the binding protein is capable of binding IL-1β and
IL-17, wherein(i) the variable domains that form a functional target binding site for IL-16 comprise; CDRs 1-3 from SEQ ID NO;
32 and CDRs 1-3 from SEQ ID NO;
33,CDRs 1-3 from SEQ ID NO;
34 and CDRs 1-3 from SEQ ID NO;
35,CDRs 1-3 from SEQ ID NO;
36 and CDRs 1-3 from SEQ ID NO;
37,CDRs 1-3 from SEQ ID NO;
38 and CDRs 1-3 from SEQ ID NO;
39, orCDRs 1-3 from SEQ ID NO;
40 and CDRs 1-3 from SEQ ID NO;
41;and (ii) the variable domains that form a functional target binding site for IL-17 comprise CDRs 1-3 from SEQ ID NO;
44 and CDRs 1-3 from SEQ ID NO;
45.- View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33)
-
Specification