Method of reducing curvature in a meniscus of liquid medium
First Claim
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1. A vessel for holding liquid, the vessel comprising:
- a) a bottom wall and a sidewall extending from the bottom wall, the sidewall and bottom wall configured so that the vessel is of integral, one-piece construction;
b) a coating material on the interior of the sidewall;
wherein the bottom wall is free from the coating material and the coating material provides a treated intrinsic contact angle between the coated interior vessel wall and an aqueous liquid within the vessel and a treated receding contact angle between the coated interior vessel wall and the aqueous liquid, the treated receding contact angle being between about 75 degrees to about 110 degrees and being closer to 90 degrees than the treated intrinsic contact angle.
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Abstract
The present application is directed to methods of improving cell culture vessel assays. In one aspect the application is directed to a method of reducing the curvature of the meniscus comprising applying a coating material to the interior wall of the vessel, wherein the coating material provides a receding contact angle of about 90 degrees with aqueous solutions and culture media. In another aspect, the application is directed to a method of labeling cells in a first solution by generating droplets of a second solution containing at least one cell-labelling agent and allowing the droplets of the second solution to contact the surface of the first solution.
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Citations
18 Claims
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1. A vessel for holding liquid, the vessel comprising:
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a) a bottom wall and a sidewall extending from the bottom wall, the sidewall and bottom wall configured so that the vessel is of integral, one-piece construction; b) a coating material on the interior of the sidewall; wherein the bottom wall is free from the coating material and the coating material provides a treated intrinsic contact angle between the coated interior vessel wall and an aqueous liquid within the vessel and a treated receding contact angle between the coated interior vessel wall and the aqueous liquid, the treated receding contact angle being between about 75 degrees to about 110 degrees and being closer to 90 degrees than the treated intrinsic contact angle. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification