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Differentiation of primate pluripotent stem cells to cardiomyocyte-lineage cells

  • US 9,062,289 B2
  • Filed: 06/20/2006
  • Issued: 06/23/2015
  • Est. Priority Date: 06/22/2005
  • Status: Active Grant
First Claim
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1. A method for obtaining a population of cells comprising α

  • myosin heavy chain (α

    MHC) expressing cells from human embryonic stem (hES) cells by direct differentiation, comprising a) culturing the hES cells in the presence of Activin A in the absence of a BMP;

    b) subsequently culturing the cells in the presence of a BMP and in the absence of Activin A for between three and five days, wherein the BMP is selected from the group consisting of BMP-2 and BMP-4 and c) subsequently culturing the cells in the presence of an insulin like growth factor (IGF) in the absence of Activin A or a BMP, wherein the IGF is selected from the group consisting of IGF-I and IGF-II, thereby producing a cell population comprising α

    myosin heavy chain (α

    MHC) expressing cells.

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