Herbicide tolerant soybean plants and methods for identifying same

• US 9,062,324 B2
• Filed: 11/23/2010
• Issued: 06/23/2015
• Est. Priority Date: 11/23/2009
• Status: Active Grant

• Current Assignee: BASF Agricultural Solutions Seed US LLC (BASF SE), MS Technologies LLC
• Original Assignee: Bayer Cropscience NV (Bayer AG), MS Technologies LLC
• Inventors: Mason, Justin Thomas, Lettow, Leslie James, Eby, Mark Alan, Eby, William H., Welz, Gnter, Verhaeghe, Steven, De Beuckeleer, Marc, Habex, Veerle, Ferrulo, Jean-Marc
• Primary Examiner(s): Kruse, David H
• Assistant Examiner(s): KOVALENKO, MYKOLA V
• Application Number:
  US12/953,251
• Publication Number:
  US 20110239321A1
• Time in Patent Office: 1,673 Days
• Field of Search: 800/300
• US Class Current: 1/1
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae

Abstract

The invention provides specific transgenic soybean plants, plant material and seeds, characterized in that these products harbor a stack of specific transformation events at specific locations in the soybean genome (elite event EE-GM3 and elite event EE-GM2, or elite event EE-GM3 and elite event EE-GM1). The invention also provides for methods of producing soybean plants and seeds having elite event EE-GM3 and elite event EE-GM2, or elite event EE-GM3 and elite event EE-GM1.

Claims

1. A plant, cell, plant part, tissue, seed, or progeny thereof, which is

(a) a soybean plant, cell, a plant part, tissue, seed, or progeny thereof, each comprising elite event EE-GM3 and elite event EE-GM1, reference seed comprising said elite event EE-GM3 having been deposited at the NCIMB under deposit number NCIMB 41659 and reference seed comprising said elite event EE-GM1 having being deposited at the NCIMB under deposit number NCIMB 41658, or reference seed comprising elite event EE-GM3 and elite event EE-GM1 in its genome having been deposited at the ATCC under deposit number PTA-11041;

or

(b) a soybean plant, plant part, seed, cell, or tissue thereof, each comprising elite event EE-GM3 and elite event EE-GM1, said soybean plant being obtained by crossing a soybean plant comprising elite event EE-GM3 obtainable by propagation of, breeding with, or propagation of and breeding with a soybean plant grown from the seed deposited at the NCIMB under deposit number NCIMB 41659 with a soybean plant comprising elite event EE-GM1 in its genome, obtainable by propagation of, breeding with, or propagation of and breeding with a soybean plant grown from the seed deposited at the NCIMB under deposit number NCIMB 41658, or said soybean plant being obtainable by propagation of, breeding with, or propagation of and breeding with a soybean plant grown from the seed deposited at the ATCC under accession number PTA-11041;

or

(c) a soybean seed comprising elite events EE-GM3 and EE-GM1, reference seed comprising elite event EE-GM3 having been deposited at the NCIMB under deposit number NCIMB 41659, reference seed comprising elite event EE-GM1 having been deposited at the NCIMB under deposit number NCIMB 41658, and reference seed comprising elite events EE-GM3 and EE-GM1 having been deposited at the ATCC under deposit number PTA-11041;

or

(d) a soybean plant, plant part, cell, or tissue, each comprising elite events EE-GM3 and EE-GM1, obtainable from the seed of (c);

or

(e) a soybean plant, or cells, part, seed or progeny thereof, each comprising elite events EE-GM3 and EE-GM1 , wherein the inserted foreign DNA of elite event EE-GM3 is oriented as indicated in FIG. 1, and wherein said event has 5′ and

3′

flanking DNA and sequences in the foreign DNA contiguous therewith, wherein said 5′

flanking DNA and the foreign DNA contiguous therewith comprises a nucleotide sequence of SEQ ID No;

2, and said 3′

flanking DNA and the foreign DNA contiguous therewith comprises a nucleotide sequence of SEQ ID No;

3, said inserted foreign DNA of FIG. 1 being present in the soybean elite event EE-GM3 deposited under deposit number NCIMB 41659, and wherein the inserted foreign DNA of elite event EE-GM1 is oriented as indicated in FIG. 3, and wherein said event has 5′ and

3′

flanking DNA and sequences in the foreign DNA contiguous therewith, wherein said 5′

flanking DNA and the foreign DNA contiguous therewith comprises a nucleotide sequence of SEQ ID No;

12, and said 3′

flanking DNA and the foreign DNA contiguous therewith comprises a nucleotide sequence of SEQ ID No;

13, said inserted foreign DNA of FIG. 3 being present in the soybean elite event EE-GM2 deposited under deposit number NCIMB 41658, or

(f) a soybean plant, plant cell, tissue, or seed, each comprising in their genome a nucleic acid molecule comprising a nucleotide sequence of SEQ ID No;

1 from nucleotide position 188 to nucleotide position 7252 or the complement thereof, and comprising in their genome a nucleic acid molecule comprising a nucleotide sequence of elite event EE-GM1 or the complement thereof, reference seed comprising elite event EE-GM1 having been deposited under deposit number NCIMB 41658, wherein said nucleotide sequence of elite event EE-GM1 comprises or is the foreign DNA sequence in SEQ ID No;

12 or 13, or the foreign DNA sequence in the plant genome between the sequence of SEQ ID No;

12 and that of SEQ ID No;

13.

2. The soybean plant, seed, cell, tissue, part or progeny of claim 1(a), the genomic DNA of which, when amplified with two primers comprising the nucleotide sequence of SEQ ID No:

4 and SEQ ID No;

5 respectively, yields a DNA fragment of about 263 bp and the genomic DNA of which, when amplified with two primers comprising the nucleotide sequence of SEQ ID No;

16 and SEQ ID No;

17 respectively, yields a DNA fragment of about 183 bp.

3. A method for producing a soybean plant or seed comprising elite events EE-GM3 and EE-GM1 comprising crossing a plant according to claim 1(a) with another soybean plant, and planting the seed comprising said elite events obtained from said cross.

9. The soybean plant, cell, part, tissue, seed or progeny of claim 1(a).

32. A method for controlling weeds in a field of soybean plants comprising elite events EE-GM3 and EE-GM1 of claim 1(a) comprising treating the field with an effective amount of an isoxaflutole-based and/or glyphosate-based and/or glufosinate-based herbicide, wherein such plants are tolerant to such herbicide(s).

33. A process for weed control, comprising treating a field in which seeds containing elite events EE-GM3 and EE-GM1 of claim 1(a) were sown with an HPPD inhibitor herbicide, optionally followed by application of glyphosate and/or glufosinate or a mixture of an HPPD inhibitor with glyphosate and/or glufosinate as post-emergent herbicide over the top of the plants.

34. A process for weed control, comprising treating soybean plants containing elite events EE-GM3 and EE-GM1 of claim 1(a) with an HPPD inhibitor herbicide over the top of the plants, applied together, followed by or preceded by a treatment with glyphosate and/or glufosinate as post-emergent herbicide over the top of the plants.

35. A method for producing a soybean plant tolerant to glyphosate and/or glufosinate and/or isoxaflutole herbicides, comprising introducing into the genome of such plant elite event EE-GM3 and elite event EE-GM1 of claim 1(a).

56. The soybean plant of claim 1(a), treated with any one of the following insecticides, herbicides or fungicides:

(a) Soybean Herbicides;

Alachlor, Bentazone, Trifluralin, Chlorimuron-Ethyl, Cloransulam-Methyl, Fenoxaprop, Fomesafen, Fluazifop, Glyphosate, Imazamox, Imazaquin, Imazethapyr, (S-)Metolachlor, Metribuzin, Pendimethalin, Tepraloxydim, Isoxaflutole, or Glufosinate;

(b) Soybean Insecticides;

Lambda-cyhalothrin, Methomyl, Parathion, Thiocarb, Imidacloprid, Clothianidin, Thiamethoxam, Thiacloprid, Acetamiprid, Dinetofuran, Flubendiamide, Rynaxypyr, Cyazypyr, Spinosad, Spinotoram, Emamectin-Benzoate, Fipronil, Ethiprole, Deltamethrin, β

-Cyfluthrin, gamma and lambda Cyhalothrin, 4-[[(6 Chlorpyridin-3-yl)methyl](2,2-difluorethy)amino]furan-2(5H)-on, Spirotetramat, Spinodiclofen, Triflumuron, Flonicamid, Thiodicarb, or beta-Cyfluthrin;

(c) Soybean Fungicides;

Azoxystrobin, Cyproconazole, Epoxiconazole, Flutriafol, Pyraclostrobin, Tebuconazole, Trifloxystrobin, Prothioconazole, or Tetraconazole.

60. The soybean plant, or cell, part, tissue, seed or progeny of claim 1(a).

61. The soybean plant, plant part, or seed, cell or tissue of claim 1(b).

62. The soybean seed of claim 1(c).

63. The soybean plant, plant part, cell, or tissue of claim 1(d).

64. The soybean plant, or cells, part, seed or progeny of claim 1(e).

65. The soybean plant, plant cell, tissue, or seed of claim 1(f).

4. A soybean plant, cell, plant part, seed, or progeny thereof, each comprising in its genome elite event EE-GM3, wherein said event comprises in order the following nucleotide sequences:

(a) the nucleotide sequence of SEQ ID No;

2 from nucleotide 1 to 1451;

(b) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID No;

1 from nucleotide 6760 to nucleotide 6958;

(c) the nucleotide sequence of SEQ ID No;

1 from nucleotide 6874 to nucleotide 7298;

(d) the nucleotide sequence of SEQ ID No;

1 from nucleotide 7 to nucleotide 7291;

(e) the nucleotide sequence of SEQ ID No;

1 from nucleotide 12 to nucleotide 7265;

(f) the nucleotide sequence of SEQ ID No;

3 from nucleotide 217 to nucleotide 240;

(g) the nucleotide sequence of SEQ ID No;

3 from nucleotide 241 to nucleotide 1408; and

further comprising in its genome elite event EE-GM1, wherein said event comprises in order the following sequences;

(h) the nucleotide sequence of SEQ ID No;

12 from nucleotide 1 to 209;

(i) the nucleotide sequence of SEQ ID No;

11 from nucleotide 340 to nucleotide 3461;

(j) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID No;

11 from nucleotide 1 to nucleotide 336;

(k) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID No;

11 from nucleotide 3462 to nucleotide 4076;

(l) the nucleotide sequence of SEQ ID No;

11 from nucleotide 337 to nucleotide 3043;

(m) the nucleotide sequence of SEQ ID No;

13 from nucleotide 559 to nucleotide 568; and

(n) the nucleotide sequence of SEQ ID No;

13 from nucleotide 569 to nucleotide 1000.

5. A plant, cell, plant part, tissue, seed, or progeny thereof, which is

(a) a soybean plant, cell, plant part, tissue, seed, or progeny thereof, each comprising elite event EE-GM3 and elite event EE-GM2 in its genome, reference seed comprising said elite event EE-GM3 having been deposited at the NCIMB under deposit number NCIMB 41659 and reference seed comprising said elite event EE-GM2 having been deposited at the NCIMB under deposit number NCIMB 41660, or reference seed comprising elite event EE-GM3 and elite event EE-GM2 in its genome having been deposited at the ATCC under deposit number PTA-11042;

or

(b) a soybean plant, plant part, tissue, seed or progeny thereof, each comprising elite event EE-GM3 and elite event EE-GM2 in its genome, said soybean plant being obtained by crossing a soybean plant comprising elite event EE-GM3 in its genome, obtainable by propagation of, breeding with, or propagation of and breeding with a soybean plant grown from the seed deposited at the NCIMB under deposit number NCIMB 41659 with a soybean plant comprising elite event EE-GM2 in its genome, obtainable by propagation of, breeding with, or propagation of and breeding with a soybean plant grown from the seed deposited at the NCIMB under deposit number NCIMB 41660, or said soybean plant being obtainable by propagation of, breeding with, or propagation of and breeding with a soybean plant grown from the seed deposited at the ATCC under accession number PTA-11042;

or

(c) a soybean seed comprising elite events EE-GM3 and EE-GM2, reference seed comprising elite event EE-GM3 having been deposited at the NCIMB under deposit number NCIMB 41659, reference seed comprising elite event EE-GM2 having been deposited at the NCIMB under deposit number NCIMB 41660, and reference seed comprising elite events EE-GM3 and EE-GM2 having been deposited at the ATCC under deposit number PTA-11042;

or

(d) a soybean plant, cell, plant part, tissue, or progeny therof, each comprising elite events EE-GM3 and EE-GM2, produced from the seed of (c);

or

(e) a soybean plant, cell, plant part, tissue, seed, or progeny thereof, each comprising elite events EE-GM3 and EE-GM1, wherein said events comprise a nucleic acid sequence of SEQ ID No;

2 from nucleotide 1431 to 1472 and a nucleic acid sequence of SEQ ID No;

3 from nucleotide 220 to 261, or the complement of said sequences, and also a nucleic acid sequence of SEQ ID No;

12 from nucleotide 199 to 220 and a nucleic acid sequence of SEQ ID No;

13 from nucleotide 558 to 579, or the complement of said sequences;

or

(f) a soybean plant, cell, plant part, tissue, seed, or progeny thereof, each comprising elite events EE-GM3 and EE-GM2, wherein said events comprise a nucleic acid sequence of SEQ ID No;

2 from nucleotide 1431 to 1472 and a nucleic acid sequence of SEQ ID No;

3 from nucleotide 220 to 261, or the complement of said sequences, and also a nucleic acid sequence of SEQ ID No;

14 from nucleotide 301 to 322 and a nucleic acid sequence of SEQ ID No;

15 from nucleotide 497 to 518, or the complement of said sequences;

or

(g) a soybean plant, cell, plant part, tissue, seed, or progeny therof, each comprising in their genome a nucleic acid molecule comprising a nucleotide sequence of SEQ ID No;

20 from nucleotide position 1452 to nucleotide position 16638 or the complement thereof, or a nucleotide sequence of SEQ ID No;

20 or the complement thereof, and comprising in their genome a nucleic acid molecule comprising a nucleotide sequence of elite events EE-GM1 or EE-GM2 or the complement thereof, reference seed comprising elite event EE-GM1 having been deposited under deposit number NCIMB 41658, and reference seed comprising elite event EE-GM2 having been deposited under deposit number NCIMB 41660, wherein said nucleotide sequence of elite events EE-GM1 or EE-GM2 comprises or is the foreign DNA sequence in SEQ ID No;

12 or 13, or the foreign DNA sequence in SEQ ID No;

14 or 15, respectively, or the foreign DNA sequence in the plant genome between the sequence of SEQ ID No;

12 and that of SEQ ID No;

13, or the foreign DNA sequence in the plant genome between the sequence of SEQ ID No;

14 and that of SEQ ID No;

15, respectively;

or

(h) a soybean plant, cell, plant part, tissue, seed, or progeny thereof, each comprising elite events EE-GM3 and EE-GM2 in its genome, wherein the inserted foreign DNA of elite event EE-GM3 is oriented as indicated in FIG. 1, and wherein said event has 5′ and

3′

flanking DNA and foreign DNA contiguous therewith, wherein said 5′

flanking DNA and the foreign DNA contiguous therewith comprises a nucleotide sequence of SEQ ID No;

2, and said 3′

flanking DNA and the foreign DNA contiguous therewith comprises a nucleotide sequence of SEQ ID No;

3, said inserted foreign DNA of FIG. 1 being present in the soybean elite event EE-GM3 deposited under deposit number NCIMB 41659, and wherein the inserted foreign DNA of elite event EE-GM2 is oriented as indicated in FIG. 4, and wherein said event has 5′ and

3′

flanking DNA and foreign DNA contiguous therewith, wherein said 5′

flanking DNA and the foreign DNA contiguous therewith comprises a nucleotide sequence of SEQ ID No;

14 and said 3′

flanking DNA and the foreign DNA contiguous therewith comprises a nucleotide sequence of SEQ ID No;

15, said inserted foreign DNA of FIG. 4 being present in the soybean elite event EE-GM2 deposited under deposit number NCIMB 41660, or

(i) a soybean plant, cell, plant part, tissue, seed, or progeny thereof, each comprising in their genome a nucleic acid molecule comprising a nucleotide sequence of SEQ ID No;

1 from nucleotide position 188 to nucleotide position 7252 or the complement thereof, and comprising in their genome a nucleic acid molecule comprising a nucleotide sequence of elite event EE-GM2 or the complement thereof, reference seed comprising elite event EE-GM2 having been deposited under deposit number NCIMB 41660, wherein said nucleotide sequence of elite event EE-GM2 comprises or is the foreign DNA sequence in SEQ ID No;

14 or 15, or the foreign DNA sequence in the plant genome between the sequence of SEQ ID No;

14 and that of SEQ ID No;

15.

6. The soybean plant, cell, plant part, tissue, seed, or progeny of claim 5(a), the genomic DNA of which, when amplified with two primers comprising the nucleotide sequence of SEQ ID No:

4 and SEQ ID No;

5 respectively, yields a DNA fragment of about 263 by and the genomic DNA of which, when amplified with two primers comprising the nucleotide sequence of SEQ ID No;

18 and SEQ ID No;

19 respectively, yields a DNA fragment of about 151 bp.

7. A method for producing a soybean plant or seed comprising elite events EE-GM3 and EE-GM2 said method comprising crossing a plant according to claim 5(a) with another soybean plant, and planting the seed comprising said elite events obtained from said cross.

10. The soybean plant, cell, plant part, tissue, seed, or progeny of claim 5(a).

11. The soybean plant, cell, plant part, tissue, seed, or progeny of claim 5(a), wherein said elite event EE-GM3 comprises a foreign DNA comprising a chimeric gene encoding the 4-hydroxyphenylpyruvate dioxygenase of Pseudomonas fluorescens strain A32 modified by the replacement of the amino acid Glycine 336 with a Tryptophane (HPPD PF W366 gene) and a chimeric gene encoding the 5-enolpyruvyl shikimate-3-phosphate synthase enzyme double mutant from Zea Mays (2mEPSPS gene), said elite event EE-GM3 also comprising nucleotides 1 to 1451 of SEQ ID No:

2 immediately upstream of and contiguous with said foreign DNA and nucleotides 241 to 1408 of SEQ ID No;

3 immediately downstream of and contiguous with said foreign DNA, and wherein said elite event EE-GM2 comprises a foreign DNA comprising a chimeric phosphinothricin acetyltransferase encoding gene, said elite event EE-GM2 also comprising nucleotides 1 to 311 of SEQ ID No;

14 immediately upstream of and contiguous with said foreign DNA and nucleotides 508 to 1880 of SEQ ID No;

15 immediately downstream of and contiguous with said foreign DNA.

12. The soybean plant, cell, plant part, tissue, seed, or progeny of claim 5(h).

13. The soybean seed of claim 5(c).

14. The soybean plant, cell, plant part, tissue, seed, or progeny of claim 5(d).

15. The soybean plant, cell, plant part, tissue, seed, or progeny of claim 5(e).

16. The soybean plant, cell, plant part, tissue, seed, or progeny of claim 5(f).

17. The soybean plant, cell, plant part, tissue, seed, or progeny of claim 5(g).

18. The soybean plant, cell, plant part, tissue, seed, or progeny of claim 5(i), comprising in their genome a nucleic acid molecule comprising a nucleotide sequence of SEQ ID No:

20 or SEQ ID No;

20 from nucleotide position 1452 to nucleotide position 16638 or the complement thereof.

19. A method for producing a soybean plant or seed comprising elite events EE-GM3 and EE-GM2 comprising crossing a plant according to claim 5(h) with another soybean plant, and planting the seed comprising elite events EE-GM3 and EE-GM2 obtained from said cross.

20. A method for producing a soybean plant or seed comprising elite events EE-GM3 and EE-GM2 comprising crossing a plant according to claim 5(f) with another soybean plant, and planting the seed comprising elite events EE-GM3 and EE-GM2 obtained from said cross.

21. A soybean product produced from seed comprising elite events EE-GM3 and EE-GM2 of any one of claim 5(a), 5(h) or 5(f), wherein said soybean product is or comprises soybean meal, ground seeds, flour, or flakes.

22. A soybean product produced from seed comprising elite events EE-GM3 and EE-GM2 of claim 6, wherein said soybean product is or comprises soybean meal, ground seeds, flour, or flakes.

24. A soybean product produced from seed comprising elite events EE-GM3 and EE-GM2 of claim 18, wherein said soybean product is or comprises soybean meal, ground seeds, flour, or flakes.

25. A method for producing a soybean product, comprising obtaining soybean seed comprising elite event EE-GM3 and elite event EE-GM2 of claim 5(a), and producing such soybean product therefrom.

26. A method for producing a soybean product, comprising obtaining soybean seed comprising elite event EE-GM3 and elite event EE-GM2 of claim 5(h), and producing such soybean product therefrom.

27. A method for producing a soybean product, comprising obtaining soybean seed comprising elite event EE-GM3 and elite event EE-GM2 of claim 5(f), and producing such soybean product therefrom.

28. A method for controlling weeds in a field of soybean plants comprising elite events EE-GM3 and EE-GM2 of claim 5(a), comprising treating the field with an effective amount of an isoxaflutole-based and/or glyphosate-based and/or glufosinate-based herbicide, wherein such plants are tolerant to such herbicide(s).

29. A process for weed control, comprising treating a field in which seeds containing elite events EE-GM3 and EE-GM2 of claim 5(a) were sown with an HPPD inhibitor herbicide, optionally followed by application of glyphosate and/or glufosinate or a mixture of an HPPD inhibitor with glyphosate and/or glufosinate as post-emergent herbicide over the top of the plants.

30. A process for weed control, comprising treating soybean plants containing elite events EE-GM3 and EE-GM2 of claim 5(a) with an HPPD inhibitor herbicide over the top of the plants, applied together, followed by or preceded by a treatment with glyphosate and/or glufosinate as post-emergent herbicide over the top of the plants.

31. A method for producing a soybean plant tolerant to glyphosate and/or glufosinate and/or isoxaflutole herbicides, comprising introducing into the genome of such plant elite event EE-GM3 and elite event EE-GM2 of claim 5(a).

57. The soybean plant of claim 5(a), treated with any one of the following insecticides, herbicides or fungicides:

(a) Soybean Herbicides;

Alachlor, Bentazone, Trifluralin, Chlorimuron-Ethyl, Cloransulam-Methyl, Fenoxaprop, Fomesafen, Fluazifop, Glyphosate, Imazamox, Imazaquin, Imazethapyr, (S-)Metolachlor, Metribuzin, Pendimethalin, Tepraloxydim, Isoxaflutole, or Glufosinate;

(b) Soybean Insecticides;

Lambda-cyhalothrin, Methomyl, Parathion, Thiocarb, Imidacloprid, Clothianidin, Thiamethoxam, Thiacloprid, Acetamiprid, Dinetofuran, Flubendiamide, Rynaxypyr, Cyazypyr, Spinosad, Spinotoram, Emamectin-Benzoate, Fipronil, Ethiprole, Deltamethrin, β

-Cyfluthrin, gamma and lambda Cyhalothrin, 4-[[(6-Chlorpyridin -3-yl)methyl](2,2-difluorethy)amino]furan-2(5H)-on, Spirotetramat, Spinodiclofen, Triflumuron, Flonicamid, Thiodicarb, or beta-Cyfluthrin;

(c) Soybean Fungicides;

Azoxystrobin, Cyproconazole, Epoxiconazole, Flutriafol, Pyraclostrobin, Tebuconazole, Trifloxystrobin, Prothioconazole, or Tetraconazole.

8. A soybean plant, cell, plant part, seed, or progeny thereof, each comprising elite event EE-GM3, wherein said event comprises in order the following nucleotide sequences:

(a) the nucleotide sequence of SEQ ID No;

2 from nucleotide 1 to 1451;

(b) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID No;

1 from nucleotide 6760 to nucleotide 6958;

(c) the nucleotide sequence of SEQ ID No;

1 from nucleotide 6874 to nucleotide 7298;

(d) the nucleotide sequence of SEQ ID No;

1 from nucleotide 7 to nucleotide 7291;

(e) the nucleotide sequence of SEQ ID No;

1 from nucleotide 12 to nucleotide 7265;

(f) the nucleotide sequence of SEQ ID No;

3 from nucleotide 217 to nucleotide 240; and

(g) the nucleotide sequence of SEQ ID No;

3 from nucleotide 241 to nucleotide 1408, and further comprising in its genome elite event EE-GM2, wherein said event comprises in order the following sequences;

(h) the nucleotide sequence of SEQ ID No;

14 from nucleotide 1 to 311;

(i) the nucleotide sequence of SEQ ID No;

11 from nucleotide 3458 to nucleotide 3848;

(j) the nucleotide sequence of SEQ ID No;

11 from nucleotide 413 to nucleotide 3457;

(k) the nucleotide sequence of SEQ ID No;

15 from nucleotide 508 to nucleotide 1880.

23. A soybean product produced from seed comprising elite events EE-GM3 and EE-GM2 of claim 8, wherein said soybean product is or comprises soybean meal, ground seeds, flour, or flakes.

36. A soybean plant, plant part, cell, tissue, or seed comprising elite events EE-GM3 and EE-GM1.

37. A method for producing a soybean plant or seed comprising elite events EE-GM3 and EE-GM1, said method comprising crossing the plant according to claim 36 with another soybean plant, and planting the seed comprising elite events EE-GM3 and EE-GM1 obtained from said cross.

38. A soybean product produced from the seed of claim 36, wherein said soybean product comprises elite events EE-GM3 and EE-GM1 and comprises soybean meal, ground seeds, soybean flour, or soybean flakes.

39. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising introducing into the genome of said soybean plant elite events EE-GM3 and EE-GM1 of claim 36, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite events EE-GM3 and EE-GM1.

40. A method for controlling weeds in a field of the soybean plant of claim 36, comprising treating the field with an effective amount of an isoxaflutole-based, glyphosate-based and/or glufosinate-based herbicide, wherein said soybean plant is tolerant to said isoxaflutole-based herbicide.

41. A method for controlling weeds in a field of the soybean plants or seeds of claim 36, comprising treating a field with an HPPD inhibitor herbicide, before said soybean plants are planted or the seeds are sown, followed by planting or sowing of said soybeans in that same pre-treated field.

42. The method of claim 41, wherein glyphosate, or an HPPD inhibitor-glyphosate mixture, is applied as post-emergent herbicide over the top of said soybean plants once weeds tend to re-appear.

43. A method for controlling weeds in a field of the soybean seeds of claim 36, comprising treating a field in which said seeds were sown, with an HPPD inhibitor herbicide before the soybean plants emerge but after the seeds are sown.

44. The method of claim 43, wherein glyphosate, or an HPPD inhibitor-glyphosate mixture, is applied as post-emergent herbicide over the top of said soybean plants once weeds tend to re-appear.

45. A method for controlling weeds in a field of the soybean plants of claim 36, comprising treating said plants with an HPPD inhibitor herbicide over the top of the soybean plants, which application can be together with, followed by or preceded by a treatment with glyphosate as post-emergent herbicide over the top of the plants.

58. The soybean plant of claim 36, treated with any one of the following insecticides, herbicides or fungicides:

(a) Soybean Herbicides;

Alachlor, Bentazone, Trifluralin, Chlorimuron-Ethyl, Cloransulam-Methyl, Fenoxaprop, Fomesafen, Fluazifop, Glyphosate, Imazamox, Imazaquin, Imazethapyr, (S-)Metolachlor, Metribuzin, Pendimethalin, Tepraloxydim, Isoxaflutole, or Glufosinate;

(b) Soybean Insecticides;

Lambda-cyhalothrin, Methomyl, Parathion, Thiocarb, Imidacloprid, Clothianidin, Thiamethoxam, Thiacloprid, Acetamiprid, Dinetofuran, Flubendiamide, Rynaxypyr, Cyazypyr, Spinosad, Spinotoram, Emamectin-Benzoate, Fipronil, Ethiprole, Deltamethrin,β

-Cyfluthrin, gamma and lambda Cyhalothrin, 4-[[(6-Chlorpyridin -3-yl)methyl](2,2-difluorethy)amino]furan-2(5H)-on, Spirotetramat, Spinodiclofen, Triflumuron, Flonicamid, Thiodicarb, or beta-Cyfluthrin;

(c) Soybean Fungicides;

Azoxystrobin, Cyproconazole, Epoxiconazole, Flutriafol, Pyraclostrobin, Tebuconazole, Trifloxystrobin, Prothioconazole, or Tetraconazole.

46. A soybean plant, plant part, cell, tissue, or seed comprising elite events EE-GM3 and EE-GM2.

47. A method for producing a soybean plant or seed comprising elite events EE-GM3 and EE-GM2, said method comprising crossing the plant according to claim 46 with another soybean plant, and planting the seed comprising elite events EE-GM3 and EE-GM2 obtained from said cross.

48. A soybean product produced from the seed of claim 46, wherein said soybean product comprises elite events EE-GM3 and EE-GM2 and comprises soybean meal, ground seeds, soybean flour, or soybean flakes.

49. A method for producing a soybean plant tolerant to glyphosate and/or isoxaflutole herbicides, comprising introducing into the genome of said soybean plant elite events EE-GM3 and EE-GM2 of claim 46, and selecting a progeny plant tolerant to glyphosate and/or isoxaflutole, wherein said progeny plant comprises elite events EE-GM3 and EE-GM2.

50. A method for controlling weeds in a field of the soybean plant of claim 46, comprising treating the field with an effective amount of an isoxaflutole-based herbicide, wherein said soybean plant is tolerant to said isoxaflutole-based herbicide.

51. A method for controlling weeds in a field of the soybean plants or seeds of claim 46, comprising treating a field with an HPPD inhibitor herbicide, before said soybean plants are planted or the seeds are sown, followed by planting or sowing of said soybeans in that same pre-treated field.

52. The method of claim 51, wherein glyphosate, or an HPPD inhibitor-glyphosate mixture, is applied as post-emergent herbicide over the top of said soybean plants once weeds tend to re-appear.

53. A method for controlling weeds in a field of the soybean seeds of claim 46, comprising treating a field in which said seeds were sown, with an HPPD inhibitor herbicide before the soybean plants emerge but after the seeds are sown.

54. The method of claim 53, wherein glyphosate, or an HPPD inhibitor-glyphosate mixture, is applied as post-emergent herbicide over the top of said soybean plants once weeds tend to re-appear.

55. A method for controlling weeds in a field of the soybean plants of claim 46, comprising treating said plants with an HPPD inhibitor herbicide over the top of the soybean plants, which application can be together with, followed by or preceded by a treatment with glyphosate as post-emergent herbicide over the top of the plants.

59. The soybean plant of claim 46, treated with any one of the following insecticides, herbicides or fungicides:

(a) Soybean Herbicides;

Alachlor, Bentazone, Trifluralin, Chlorimuron-Ethyl, Cloransulam-Methyl, Fenoxaprop, Fomesafen, Fluazifop, Glyphosate, Imazamox, Imazaquin, Imazethapyr, (S-)Metolachlor, Metribuzin, Pendimethalin, Tepraloxydim, Isoxaflutole, or Glufosinate;

(b) Soybean Insecticides;

Lambda-cyhalothrin, Methomyl, Parathion, Thiocarb, Imidacloprid, Clothianidin, Thiamethoxam, Thiacloprid, Acetamiprid, Dinetofuran, Flubendiamide, Rynaxypyr, Cyazypyr, Spinosad, Spinotoram, Emamectin-Benzoate, Fipronil, Ethiprole, Deltamethrin, β

-Cyfluthrin, gamma and lambda Cyhalothrin, 4-[[(6-Chlorpyridin -3-yl)methyl](2,2-difluorethyeamino]furan-2(5H)-on, Spirotetramat, Spinodiclofen, Triflumuron, Flonicamid, Thiodicarb, or beta-Cyfluthrin;

(c) Soybean Fungicides;

Azoxystrobin, Cyproconazole, Epoxiconazole, Flutriafol, Pyraclostrobin, Tebuconazole, Trifloxystrobin, Prothioconazole, or Tetraconazole.