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Transcriptional profiling of stem cells and their multilineage differentiation

  • US 9,062,346 B2
  • Filed: 12/15/2010
  • Issued: 06/23/2015
  • Est. Priority Date: 04/01/2005
  • Status: Active Grant
First Claim
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1. A method of screening a human stem cell for differentiation into an osteogenic specific cell line, comprising:

  • (a) providing a human stem cell for which differentiation is to be determined, then(b) subjecting said human stem cell to differentiating conditions; and

    then(c) detecting in said stem cell differential expression of at least four osteogenic specific genes selected from the group consisting of;

    intracellular adhesion molecule 1 (ICAM1), osteomodulin (OMD), tissue inhibitor of metalloproteinase 4 (TIMP4), sex determining region Y box 4 (SOX4), crystalin alpha B (CRYAB), secreted phosphoprotein 1 (SPP1), v-fos FBJ murine steosarcoma viral oncogene homolog (FOS), alpha V integrin (ITGAV), prolactin (PRL), alpha 4 integrin (ITGA4), peroxisome proliferative activated receptor gamma (PPARG), secreted protein, acidic, cystein-rich (SPARC), sarcoma amplified sequence (SAS), and bone morphogenetic protein 1 (BMP1),wherein upregulation of expression of said at least four osteogenic specific genes indicates differentiation of said human stem cell into an osteogenic specific cell line.

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