Method for high-throughput AFLP-based polymorphism detection
First Claim
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1. A method for genotyping genetic markers in one or more samples, comprising the steps of:
- (a) reducing complexity of at least two nucleic acid samples using amplified fragment length polymorphism (AFLP) technology to produce at least two reproducible subsets of amplified adaptor-ligated restriction fragments from the at least two nucleic acid samples, wherein each reproducible subset comprises a unique identifier tag and wherein the AFLP technology uses primers having no selective nucleotides;
(b) sequencing a pool of the at least two reproducible subsets of amplified adaptor-ligated restriction fragments using sequencing by synthesis on a solid support;
(c) aligning the sequences of the reproducible subsets of amplified adaptor-ligated restriction fragments;
(d) identifying AFLP and/or single-nucleotide polymorphism (SNP) markers within a selection of amplified adaptor-ligated restriction fragments that are sequenced with a redundancy of at least 6; and
(e) determining dominant or co-dominant genotypes of the genetic markers in the selected amplified adaptor-ligated restriction fragments that are sequenced with the redundancy of at least 6.
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Abstract
The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
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Citations
5 Claims
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1. A method for genotyping genetic markers in one or more samples, comprising the steps of:
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(a) reducing complexity of at least two nucleic acid samples using amplified fragment length polymorphism (AFLP) technology to produce at least two reproducible subsets of amplified adaptor-ligated restriction fragments from the at least two nucleic acid samples, wherein each reproducible subset comprises a unique identifier tag and wherein the AFLP technology uses primers having no selective nucleotides; (b) sequencing a pool of the at least two reproducible subsets of amplified adaptor-ligated restriction fragments using sequencing by synthesis on a solid support; (c) aligning the sequences of the reproducible subsets of amplified adaptor-ligated restriction fragments; (d) identifying AFLP and/or single-nucleotide polymorphism (SNP) markers within a selection of amplified adaptor-ligated restriction fragments that are sequenced with a redundancy of at least 6; and (e) determining dominant or co-dominant genotypes of the genetic markers in the selected amplified adaptor-ligated restriction fragments that are sequenced with the redundancy of at least 6. - View Dependent Claims (2, 3, 4, 5)
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Specification