Generation of pluripotent stem cells using recombinant proteins
First Claim
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1. A method of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell, the method comprising:
- contacting the non-pluripotent cell with a plurality of exogenous transcription factor polypeptides, each exogenous transcription factor polypeptide comprising a transcription factor fused to a heterologous peptide sequence that enhances transport across cell membranes, under conditions to produce the induced pluripotent stem cell from the mammalian non-pluripotent cell;
wherein the exogenous transcription factor polypeptides are selected from the group consisting of;
(i) Oct4, Klf4, and Sox2; and
(ii) Oct4, Klf4, Sox2, and c-Myc; and
contacting the non-pluripotent cell with a MEK inhibitor, a GSK3 inhibitor, and a TGFβ
receptor inhibitor/ALK5 inhibitor.
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Abstract
The present invention provides for methods, compositions, and kits of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell using exogenous transcription factors.
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Citations
14 Claims
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1. A method of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell, the method comprising:
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contacting the non-pluripotent cell with a plurality of exogenous transcription factor polypeptides, each exogenous transcription factor polypeptide comprising a transcription factor fused to a heterologous peptide sequence that enhances transport across cell membranes, under conditions to produce the induced pluripotent stem cell from the mammalian non-pluripotent cell; wherein the exogenous transcription factor polypeptides are selected from the group consisting of;
(i) Oct4, Klf4, and Sox2; and
(ii) Oct4, Klf4, Sox2, and c-Myc; andcontacting the non-pluripotent cell with a MEK inhibitor, a GSK3 inhibitor, and a TGFβ
receptor inhibitor/ALK5 inhibitor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification