Microorganisms expressing ketoacyl-CoA synthase and uses thereof
First Claim
1. A recombinant oleaginous cell of the genus Prototheca, the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca, wherein the cell produces an oil elevated in erucic acid due to the expression of the exogenous gene.
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Accused Products
Abstract
Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
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Citations
12 Claims
- 1. A recombinant oleaginous cell of the genus Prototheca, the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca, wherein the cell produces an oil elevated in erucic acid due to the expression of the exogenous gene.
- 7. A recombinant oleaginous cell of the genus Prototheca, the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca, wherein the cell produces an oil elevated in eicosenoic acid due to the expression of the exogenous gene.
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11. A recombinant oleaginous cell of the genus Prototheca, the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca, wherein the cell produces an oil elevated in docosadienoic acid due to the expression of the exogenous gene.
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12. A recombinant oleaginous cell of the genus Prototheca, the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca, wherein the cell produces an oil elevated in C22:
- 5 due to the expression of the exogenous gene.
Specification