TSH immunoassays employing scavenging reagents for cross-reacting endocrine glycoprotein hormone analogues
First Claim
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1. A method for performing a thyroid stimulating hormone (TSH) immunoassay, the method comprising the steps of:
- (a) inserting a fluid sample into a device comprising an immunosensor in a conduit, said immunosensor having a capture antibody to TSH;
(b) dissolving a signal antibody into said fluid sample;
(c) amending said fluid sample with scavenger beads for one or more of follicle stimulating hormone (FSH), luteinizing hormone (LH) and chorionic gonadotropin (CG);
(d) forming a sandwich complex on said immunosensor, said complex comprising said capture antibody, TSH, and said signal antibody;
(e) washing uncomplexed signal antibody from the immunosensor with a wash fluid; and
(f) detecting a signal associated with said complexed signal antibody,wherein the scavenger beads are removed to a waste chamber downstream of the immunosensor prior to the detecting.
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Abstract
Thyroid Stimulating Hormone (TSH) immunoassays are performed using an ELISA sandwich assay that employs scavenging or sacrificial beads for reducing interference caused by cross-reacting endocrine glycoprotein hormone analogs such as Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Chorionic Gonadotropin (CG).
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22 Claims
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1. A method for performing a thyroid stimulating hormone (TSH) immunoassay, the method comprising the steps of:
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(a) inserting a fluid sample into a device comprising an immunosensor in a conduit, said immunosensor having a capture antibody to TSH; (b) dissolving a signal antibody into said fluid sample; (c) amending said fluid sample with scavenger beads for one or more of follicle stimulating hormone (FSH), luteinizing hormone (LH) and chorionic gonadotropin (CG); (d) forming a sandwich complex on said immunosensor, said complex comprising said capture antibody, TSH, and said signal antibody; (e) washing uncomplexed signal antibody from the immunosensor with a wash fluid; and (f) detecting a signal associated with said complexed signal antibody, wherein the scavenger beads are removed to a waste chamber downstream of the immunosensor prior to the detecting. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification