System for controlling and optimizing reactions in solid phase synthesis of small molecules
First Claim
1. A solid phase synthesizer reaction system, of a type utilizing a plurality of flow through reaction vessels, each reaction vessel having an open top to allow introduction of reagents through said reaction vessel, an open bottom of each reaction vessel to allow liquid to flow from said reaction vessel and a solid phase reaction material within each of said reaction vessel between said open top end and open bottom end, said system comprising:
- a rotor configured to hold a plurality of reaction vessels;
a cover positioned above said rotor;
an upper sealable chamber on said rotor, said reaction vessel open tops within said upper sealable chamber;
a lower sealable chamber within said rotor, said reaction vessel open bottoms within said lower sealable chamber;
a controllable pressure regulation pathway between said upper sealable chamber and said lower sealable chamber, said controllable pressure regulation pathway allowing equalization of pressure between said upper chamber and said lower chamber without requiring that gas pass through said reaction vessels.
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Accused Products
Abstract
A instrument for performing synthesis of small molecules such as Oligos and Peptides when using solid phase synthesis techniques to synthesize small molecules. The system and methods include a mechanism for controlling the pressure differential across the reaction vessels that contain the solid support used in solid phase synthesis. Reaction vessels are held in a holder that provides a sealable chamber at the outlet ends of the reaction vessels. The rotor containing the reaction vessels is placed within a sealable chamber. The sealable chamber is fitted with a means for engaging the rotor and draining the reaction vessels to waste. The sealable chamber is also fitted with a means for engaging the rotor to drain the reaction vessels at a slower, variable rate.
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Citations
17 Claims
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1. A solid phase synthesizer reaction system, of a type utilizing a plurality of flow through reaction vessels, each reaction vessel having an open top to allow introduction of reagents through said reaction vessel, an open bottom of each reaction vessel to allow liquid to flow from said reaction vessel and a solid phase reaction material within each of said reaction vessel between said open top end and open bottom end, said system comprising:
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a rotor configured to hold a plurality of reaction vessels; a cover positioned above said rotor; an upper sealable chamber on said rotor, said reaction vessel open tops within said upper sealable chamber; a lower sealable chamber within said rotor, said reaction vessel open bottoms within said lower sealable chamber; a controllable pressure regulation pathway between said upper sealable chamber and said lower sealable chamber, said controllable pressure regulation pathway allowing equalization of pressure between said upper chamber and said lower chamber without requiring that gas pass through said reaction vessels. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A solid phase synthesizer reaction system, of a type utilizing a plurality of flow through reaction vessels, each reaction vessel having an open top to allow introduction of reagents through said reaction vessel, an open bottom of each reaction vessel to allow liquid to flow from said reaction vessel and a solid phase reaction material within each of said reaction vessel between said open top end and open bottom end, said system comprising:
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a rotor configured to hold a plurality of reaction vessels; a cover positioned above said rotor; a sealable chamber within said rotor, said reaction vessel open bottoms within said sealable chamber; a first drain connectable to said lower sealed chamber, configured to allow only gas flow from said lower sealable chamber and having a controllable flow rate regulation system; a first drain control valve, configured to open or close flow through said first drain; a second drain positioned within said lower sealable chamber such that it drains both gas and liquid from said chamber; and a second drain control valve configured to open or close flow through said second drain. - View Dependent Claims (11, 12, 13, 14)
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15. A method for control of flow through reaction vessels during solid phase synthesis reaction, wherein said reaction vessels each have an open top end, an open bottom, said reaction vessels positioned on a rotor such that said open bottom end is enclosed in a sealable chamber within said rotor, said method comprising:
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equalizing a pressure utilizing a passageway that connects between an area holding said reaction vessel open top and said sealable chamber without flow through said reaction vessel, thereby limiting flow through said reaction vessel; and Opening a variable flow rate gas drain to controllably drive fluid flow through said reaction vessels. - View Dependent Claims (16, 17)
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Specification