Digital analyte analysis
First Claim
1. A plurality of microdroplets, each comprising a single nucleic acid template and a plurality of species of primer pairs, each species of primer pairs being specific for a different target site, wherein each of the single nucleic acid templates in the microdroplets comprise one of the different target sites that is recognized by only a single species of primer pairs, wherein the single species of the primer pairs binds to the target site of the single nucleic acid template in each microdroplet.
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Abstract
The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
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Citations
9 Claims
- 1. A plurality of microdroplets, each comprising a single nucleic acid template and a plurality of species of primer pairs, each species of primer pairs being specific for a different target site, wherein each of the single nucleic acid templates in the microdroplets comprise one of the different target sites that is recognized by only a single species of primer pairs, wherein the single species of the primer pairs binds to the target site of the single nucleic acid template in each microdroplet.
Specification