Compositions and reaction mixtures for the detection of nucleic acids from multiple types of human papillomavirus
First Claim
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1. A combination of amplification oligomers for detecting human papillomavirus (HPV) of multiple HPV types, the combination comprising first amplification oligonucleotides, comprising a target binding region and a 5′
- promoter sequence, and second amplification oligonucleotides, comprising a target binding region, wherein pairs of the first and second amplification oligonucleotides can hybridize to opposing strands of HPV nucleic acid for transcription mediated amplification of HPV nucleic acid between hybridized pairs of the first and second amplification oligonucleotides, wherein(a) the target binding regions of the first amplification oligomers consist of SEQ ID Nos. 19, 21, 23, 25, 29, 31, 33, 35, and 37, respectively, and the target binding regions of the second amplification oligomers consist of SEQ ID Nos. 38, 39, 40, and 41, respectively;
or complements thereof;
RNA equivalents thereof;
or RNA equivalents of the complements thereof;
or(b) the target binding regions of the first amplification oligomers consists of SEQ ID Nos. 21, 23, 25, 29, 31, 33, 35, 37, and 42, respectively, and the target binding regions of the second amplification oligomers consist of SEQ ID Nos. 38, 39, 40, and 41, respectively, or complements thereof;
RNA equivalents thereof;
or RNA equivalents of the complements thereof.
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Abstract
Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed.
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Citations
9 Claims
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1. A combination of amplification oligomers for detecting human papillomavirus (HPV) of multiple HPV types, the combination comprising first amplification oligonucleotides, comprising a target binding region and a 5′
- promoter sequence, and second amplification oligonucleotides, comprising a target binding region, wherein pairs of the first and second amplification oligonucleotides can hybridize to opposing strands of HPV nucleic acid for transcription mediated amplification of HPV nucleic acid between hybridized pairs of the first and second amplification oligonucleotides, wherein
(a) the target binding regions of the first amplification oligomers consist of SEQ ID Nos. 19, 21, 23, 25, 29, 31, 33, 35, and 37, respectively, and the target binding regions of the second amplification oligomers consist of SEQ ID Nos. 38, 39, 40, and 41, respectively;
or complements thereof;
RNA equivalents thereof;
or RNA equivalents of the complements thereof;
or(b) the target binding regions of the first amplification oligomers consists of SEQ ID Nos. 21, 23, 25, 29, 31, 33, 35, 37, and 42, respectively, and the target binding regions of the second amplification oligomers consist of SEQ ID Nos. 38, 39, 40, and 41, respectively, or complements thereof;
RNA equivalents thereof;
or RNA equivalents of the complements thereof. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- promoter sequence, and second amplification oligonucleotides, comprising a target binding region, wherein pairs of the first and second amplification oligonucleotides can hybridize to opposing strands of HPV nucleic acid for transcription mediated amplification of HPV nucleic acid between hybridized pairs of the first and second amplification oligonucleotides, wherein
Specification