Biosensor, biosensor chip and biosensor device

• US 9,075,000 B2
• Filed: 10/08/2014
• Issued: 07/07/2015
• Est. Priority Date: 07/02/2002
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Inventors: Ueno, Hiroya, Nakatsuka, Junji
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US14/509,802
• Publication Number:
  US 20150053558A1
• Time in Patent Office: 272 Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/30 Electrodes, e.g. test electrodes; Half-cells G01N27/414 takes precedence
• CPC Class Codes: G01N 27/307 Disposable laminated or multilayered electrodes G01N27/3272 takes precedence
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3273 Devices therefor, e.g. test element readers, circuitry details not specific to biochemical electrodes G01N33/4875
• CPC Class Codes: G01N 27/416 Systems G01N27/27 takes precedence
• CPC Class Codes: G01R 19/0092 measuring current only all subgroups of G01R19/00 take precedence

Abstract

A biosensor includes a working electrode 101, a counter electrode 102 opposing the working electrode 101, a working electrode terminal 103 and a working electrode reference terminal 10 connected to the working electrode 101 by wires, and a counter electrode terminal 104 connected to the counter electrode 102 by a wire. By employing a structure with at least three electrodes, it is possible to assay a target substance without being influenced by the line resistance on the working electrode side.

Claims

1. A biosensor, comprising:

a working electrode disposed on a proximal end of the biosensor;

a counter electrode disposed on the proximal end of the biosensor;

a working electrode terminal disposed on a distal end of the biosensor;

a counter electrode terminal disposed on the distal end of the biosensor;

a first reference terminal disposed on the distal end of the biosensor;

a second reference terminal disposed on the distal end of the biosensor;

a first conductive line connecting the working electrode to the working electrode terminal;

a second conductive line connecting the working electrode to the first reference terminal;

a third conductive line connecting the counter electrode to the counter electrode terminal;

a fourth conductive line connecting the counter electrode to the second reference terminal; and

an additional counter electrode disposed on the proximal end of the biosensor, wherein the working electrode is provided between the counter electrode and the additional counter electrode.

2. The biosensor of claim 1, wherein:

the second conductive line is provided to enable a voltage at the working electrode to be detected; and

the fourth conductive line is provided to enable a voltage at the counter electrode to be detected.

3. The biosensor of claim 1, wherein the second conductive line and the fourth conductive line are provided to enable a voltage between the working electrode and the counter electrode to be constant.

4. A biosensor device, comprising:

a biosensor, comprising;

a working electrode disposed on a proximal end of the biosensor;

a counter electrode disposed on the proximal end of the biosensor;

a working electrode terminal disposed on a distal end of the biosensor;

a counter electrode terminal disposed on the distal end of the biosensor;

a first reference terminal disposed on the distal end of the biosensor;

a second reference terminal disposed on the distal end of the biosensor;

a first conductive line connecting the working electrode to the working electrode terminal;

a second conductive line connecting the working electrode to the first reference terminal;

a third conductive line connecting the counter electrode to the counter electrode terminal;

a fourth conductive line connecting the counter electrode to the second reference terminal; and

an additional counter electrode disposed on the proximal end of the biosensor, wherein the working electrode is provided between the counter electrode and the additional counter electrode; and

a measurement circuit connected to the biosensor for performing an assay on a fluid based on a current flowing through the first conductive line or the third conductive line;

wherein substantially no current flows through the second conductive line and through the fourth conductive line during the assay.