Microscopy imaging

• US 9,075,225 B2
• Filed: 04/27/2011
• Issued: 07/07/2015
• Est. Priority Date: 10/28/2009
• Status: Active Grant

• Current Assignee: Alentic Microscience Inc.
• Original Assignee: Alentic Microscience Inc.
• Inventors: Fine, Alan Marc
• Primary Examiner(s): Vaughn, Jr., William C
• Assistant Examiner(s): BELAI, NAOD W
• Application Number:
  US13/095,175
• Publication Number:
  US 20110249109A1
• Time in Patent Office: 1,532 Days
• Field of Search: 348/79
• US Class Current: 1/1
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16
• CPC Class Codes: G01N 2201/062 LED's
• CPC Class Codes: G01Q 60/20 Fluorescence
• CPC Class Codes: G02B 21/0008 Microscopes having a simple construction, e.g. portable microscopes
• CPC Class Codes: G02B 21/002 Scanning microscopes scanning near field optical microscopes G01Q60/18
• CPC Class Codes: G02B 21/16 adapted for ultraviolet illumination ; Fluorescence microscopes G02B21/0076 takes precedence
• CPC Class Codes: G02B 21/36 arranged for photographic purposes or projection purposes G02B21/18 takes precedenceor digital imaging or video purposes including associated control and data processing arrangements image data processing per se G06T
• CPC Class Codes: H01L 27/14643 Photodiode arrays; MOS imagers

Abstract

Among other things, an imaging device has a photosensitive array of pixels, and a surface associated with the array is configured to receive a specimen with at least a part of the specimen at a distance from the surface equivalent to less than about half of an average width of the pixels.

Claims

1. An apparatus comprising:

a portable housing comprising;

an imaging integrated circuit comprising

a two-dimensional array of pixels at a photosensitive surface to capture a two-dimensional image of a specimen, the width of the pixels along each of the two dimensions of the array being 5 microns or smaller, the array having at least 1 million pixels,

the surface to receive at least a part of a specimen at a distance from the surface equivalent to less than about half of an average size of the pixels,

the pixels situated within the array to generate signals that capture a two-dimensional image of the specimen that extends along each of the two dimensions of the array,

a chamber for the specimen, the chamber being defined partly by a chamber lid comprising a top, spaced apart from the surface by a distance less than 1 millimeter, to transmit light to illuminate the specimen,

the chamber lid being movable to permit a liquid specimen to be applied to the surface, or the chamber lid comprising a fluid channel to carry a fluid specimen into the chamber and in contact with the surface, or both,

the specimen to be held statically after being applied to the surface or carried into the chamber, or the two-dimensional image being captured at once as the fluid specimen flows past the surface,

electronics comprising a circuit board coupled to the imaging integrated circuit, and

a computing device to analyze information representing, or to display, the two-dimensional image of the specimen captured by the array, or both.

2. The apparatus of claim 1 also comprising a fluid channel to carry the specimen out of the chamber.

3. The apparatus of claim 2 also comprising one or more chamber lid electrodes contacting the top of the specimen chamber.

4. The apparatus of claim 3 in which the surface has a conductive layer functioning as one or more surface electrodes.

5. The apparatus of claim 4 also comprising wires electrically connecting the one or more surface electrodes and the one or more chamber lid electrodes to a potential-applying device.

6. The apparatus of claim 5 also comprising wires electrically connecting the one or more surface electrodes and the one or more chamber lid electrodes to a potential-measuring device.

7. The apparatus of claim 5 in which the potential-applying device is also capable of measuring a potential.

8. The apparatus of claim 5 in which the one or more chamber lid electrodes are arranged in a matrix and the potential-applying device is configured to address the one or more chamber lid electrodes individually or as a group.

9. The apparatus of claim 5 in which the one or more surface electrodes are arranged in a matrix and the potential-applying device is configured to address the one or more surface electrodes individually or as a group.

10. The apparatus of claim 1 comprising a light source to emit light primarily in the ultraviolet wavelength band and in which longer-wavelength fluorescence or phosphorescence of the specimen produces a signal.

11. A method of fluorescence lifetime imaging comprising operating the apparatus of claim 10.

12. A method of collecting specimen comprising operating the apparatus of claim 5 with a potential applied between the chamber lid electrode and the surface electrode.

13. A method of performing wound healing experiments comprising operating the apparatus of claim 5.

14. The apparatus of claim 1 in which the specimen is suspended in a fluid.

15. The apparatus of claim 1 comprising a collimated light source.

16. The apparatus of claim 1 comprising a layer attached to the surface of the array of photosensitive elements.

17. The portable apparatus of claim 1 in which the array has at least a million light-sensitive elements.

18. The portable apparatus of claim 1 comprising a pump for causing the specimen to flow through the chamber and the channel.