Systems, methods, compositions and solutions for perfusing an organ

• US 9,078,428 B2
• Filed: 10/07/2005
• Issued: 07/14/2015
• Est. Priority Date: 06/28/2005
• Status: Active Grant

• Current Assignee: TransMedics, Inc. (TransMedics Group, Inc.)
• Original Assignee: TransMedics, Inc. (TransMedics Group, Inc.)
• Inventors: Hassanein, Waleed, Lezberg, Paul, Khayal, Tamer
• Primary Examiner(s): Saucier, Sandra
• Application Number:
  US11/246,919
• Publication Number:
  US 20060292544A1
• Time in Patent Office: 3,567 Days
• US Class Current: 1/1
• CPC Class Codes: A01N 1/02 Preservation of living parts
• CPC Class Codes: A01N 1/0226 Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
• CPC Class Codes: A01N 1/0247 for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
• CPC Class Codes: C12M 21/08 for producing artificial tissue or for ex-vivo cultivation of tissue prostheses A61F2/00, grafts A61L27/00

Abstract

The invention, in various embodiments, provides systems, methods and solutions for perfusing an organ.

Claims

1. A composition comprising in an aqueous medium:

adenosine at a concentration of about 675 mg/1500 mL to about 825 mg/1500 mL,

calcium chloride dehydrate in an amount of about 2100 mg/1500 mL to about 2600 mg/1500 mL,

glycine in an amount of about 315 mg/1500 mL to about 385 mg/1500 mL,

L-alanine in an amount of about 150 mg/1500 mL to about 200 mg/1500 mL,

L-arginine in an amount of about 600 mg/1500 mL to about 800 mg/1500 mL,

L-aspartic acid in an amount of about 220 mg/1500 mL to about 270 mg/1500 mL,

L-glutamic acid in an amount of about 230 mg/1500 mL to about 8290 mg/1500 mL,

L-histidine in an amount of about 200 mg/1500 mL to about 250 mg/1500 mL,

L-isoleucine in an amount of about 100 mg/1500 mL to about 130 mg/1500 mL,

L-leucine in an amount of about 300 mg/1500 mL to about 380 mg/1500 mL,

L-methionine in an amount of about 50 mg/1500 mL to about 65 mg/1500 mL,

L-phenylalanine in an amount of about 45 mg/1500 mL to about 60 mg/1500 mL,

L-proline in an amount of about 110 mg/1500 mL to about 140 mg/1500 mL,

L-serine in an amount of about 80 mg/1500 mL to about 105 mg/1500 mL,

L-threonine in an amount of about 60 mg/1500 mL to about 80 mg/1500 mL,

L-tryptophan in an amount of about 30 mg/1500 mL to about 40 mg/1500 mL,

L-tyrosine in an amount of about 80 mg/1500 mL to about 110 mg/1500 mL,

L-valine in an amount of about 150 mg/1500 mL to about 190 mg/1500 mL,

lysine acetate in an amount of about 200 mg/1500 mL to about 250 mg/1500 mL,

magnesium sulfate heptahydrate in an amount of about 535 mg/1500 mL to about 635 mg/1500 mL,

potassium chloride in an amount of about 200 mg/1500 mL to about 210 mg/1500 mL,

sodium chloride in an amount of about 11.1 g/1500 mL to about 11.6 g/1500 mL,

dextrose in an amount of about 25 g/1500 mL to about 120 g/1500 mL,

epinephrine in an amount of about 0.25 mg/1500 mL to about 1.0 mg/1500 mL,

insulin in an amount of about 75 IU/1500 mL to about 150 IU/1500 mL,

one or more vitamins in an amount of about 1 mg/1500 mL to about 30 mg/1500 mL,

methylprednisolone sodium succinate in an amount of about 200 mg/1500 mL to 500 mg/1500 mL,

sodium bicarbonate in an amount of about 10 mEq/1500 mL to 25 mEq/1500 mL,

mannitol in an amount of about 12.5 g/1500 mL, and

sodium glycerophosphate in an amount of about 900 mg/1500 mL.