Protection of bioanalytical sample chambers

• US 9,079,182 B2
• Filed: 10/14/2011
• Issued: 07/14/2015
• Est. Priority Date: 04/15/2009
• Status: Active Grant

• Current Assignee: Biocartis NV (Biocartis Group NV)
• Original Assignee: Biocartis NV (Biocartis Group NV)
• Inventors: De Gier, Ronald, Versleegers, Jozef C. M.
• Primary Examiner(s): HOBBS, MICHAEL L
• Application Number:
  US13/273,533
• Publication Number:
  US 20120034687A1
• Time in Patent Office: 1,369 Days
• Field of Search: 435/63, 435/287.2, 435/6.1, 422/401, 422/402, 422/417, 422/502, 422/503
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2200/18 Transport of container or devices
• CPC Class Codes: B01L 2300/041 Connecting closures to device or container
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 9/52 Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips

Abstract

An apparatus for performing bioanalytic processing and analysis, a bioanalytical reaction device, and a cartridge thereof are provided. The cartridge contains a housing and at least one sample chamber in a platform for storing biological samples, which the bioanalytical reaction device can process and analyze. The platform is movably connected to the housing such that the platform is movable between a stowed position, in which the sample chamber is protected by the housing, and an extended position, in which the sample chamber is outside of the housing. The bioanalytical reaction device includes an actuation device for moving the platform between the stowed and extended positions.

Claims

1. A cartridge for a bioanalytical reaction device, said cartridge comprising:

a housing;

a platform having one or more sample chambers provided therein for receiving a respective sample, each said sample chamber is defined by an opening in the platform, wherein at least one of the sample chambers includes a wall that allows the sample to be processed or analyzed by the bioanalytical reaction device through the wall, wherein

the platform is moveably connected to the housing for movement of the platform relative to the housing, said platform moveable between (i) a stowed position, in which the wall is protected by the housing so that the sample cannot be processed or analyzed by the bioanalytical reaction device through the wall, and (ii) an extended position, in which the wall is outside of the housing for processing or analyzing the sample with the bioanalytical reaction device through the wall; and

an actuation device connected to the platform to move the platform between the stowed and the extended positions.

2. The cartridge of claim 1, wherein the at least one sample chamber is connected to a channel for filling the at least one sample chamber, the channel ending in the vicinity of the actuation device.

3. The cartridge of claim 2, wherein a part of the channel is located within the actuation device.

4. The cartridge of claim 1, wherein the wall is arranged at a first side of the platform, the platform having a second side opposite to the first side, and wherein the platform, when in the extended position, is accessible from the first side and the second side by the bioanalytical reaction device for processing or analyzing the sample.

5. The cartridge of claim 1, wherein at least one dimension of the cartridge with the platform in the extended position is bigger than the dimension with the platform in the stowed position.

6. The cartridge of claim 1, wherein the platform is rotatably connected to the housing.

7. The cartridge of claim 6, wherein the actuation device is a shaft and the platform is connected to the shaft for rotating the platform about a rotation axis.

8. The cartridge of claim 1, wherein the platform is slidably connected to the housing.

9. The cartridge of claim 1, wherein the platform comprises a plate having said opening formed therein, said plate arranged between a first wall of the housing and a second wall of the housing, in the stowed position.

10. The cartridge of claim 1, wherein the wall is formed by a foil that covers the opening.

24. The cartridge of claim 1, wherein said wall provides an interface for heating or cooling the at least one sample chamber.

25. The cartridge of claim 1, wherein said wall provides an optical interface allowing light to travel to and from the at least one sample chamber.

26. The cartridge of claim 1, wherein said wall is arranged at a first side of the platform, and said at least one sample chamber has a second wall at a second side of the platform opposite to the first side of the platform.

27. The cartridge of claim 1, wherein said wall provides at least one of:

(i) an interface that allows the transfer of heat therethrough, and

(ii) an optical interface that allows light to penetrate therethrough.

11. A bioanalytical reaction device, comprising:

a cartridge having;

a housing,

a platform having one or more sample chambers provided therein for receiving a respective sample, each said sample chamber is defined by an opening in the platform, wherein at least one of the sample chambers includes a wall that allows the sample to be processed or analyzed through the wall,

wherein the platform is moveably connected to the housing for movement of the platform relative to the housing, said platform moveable between (i) a stowed position, in which the wall is protected by the housing so that the sample cannot be processed or analyzed through the wall, and (ii) an extended position, in which the wall is outside of the housing for processing or analyzing the sample through the wall, and

an actuation device connected to the platform to move the platform between the stowed and the extended positions;

a slot for receiving the cartridge; and

an actuator for engaging the actuation device when the cartridge is received in the slot to move the platform between the stowed and the extended positions.

12. The bioanalytical reaction device of claim 11, wherein the actuator is a motor.

13. The bioanalytical reaction device of claim 11, wherein the cartridge further comprises:

a reservoir for filling the at least one sample chamber, wherein the reservoir is connectable, by the actuator, with the at least one sample chamber over a line extending through the actuator.

14. The bioanalytical reaction device of claim 11, further comprising a cartridge presence sensor for detecting a presence of the cartridge in the slot.

15. The bioanalytical reaction device of claim 14, wherein the actuator engages the actuation device to move the platform to the extended position when the cartridge presence sensor detects the presence of the cartridge in the slot.

16. The bioanalytical reaction device of claim 11, wherein the platform is rotatably connected to the housing.

17. The bioanalytical reaction device of claim 11, wherein the actuation device is a shaft and the platform is connected to the shaft for rotating the platform about a rotation axis.

18. The bioanalytical reaction device of claim 11, wherein the platform is slidably connected to the housing.

19. The bioanalytical reaction device of claim 11, wherein the platform comprises a plate having said opening formed therein, said plate arranged between a first wall of the housing and a second wall of the housing, in the stowed position.

20. The bioanalytical reaction device of claim 11, wherein the wall is formed by a foil that covers the opening.

28. The bioanalytical reaction device of claim 11, wherein said wall of the cartridge provides at least one of:

(i) an interface that allows the transfer of heat therethrough, and

(ii) an optical interface that allows light to penetrate therethrough.

21. A cartridge for a bioanalytical reaction device, comprising:

a housing adapted to be received in the bioanalytical reaction device;

a platform having one or more sample chambers provided therein for receiving a respective sample, each sample chamber defined by an opening in the platform, wherein at least one of the sample chambers includes a wall that allows the sample to be processed or analyzed by the bioanalytical reaction device through the wall, wherein the platform is moveably connected to the housing such that, when the housing is received in the bioanalytical reaction device, the platform is moveable between (i) a stowed position, in which the wall is inside the housing, and (ii) an extended position, in which the wall is outside of the housing, the sample of the at least one sample chamber is processed or analyzed by the bioanalytical reaction device through the wall when the platform is in the extended position; and

a shaft rotatably connecting the platform to the housing for movement of the platform between the stowed and the extended positions.

22. The cartridge of claim 21, further comprising a fluid channel defined in the shaft, the fluid channel being in fluid communication with the at least one sample chamber.

23. The cartridge of claim 21, wherein said wall is formed by a foil that covers the opening.

29. The cartridge of claim 21, wherein said wall provides at least one of:

(i) an interface that allows the transfer of heat therethrough, and

(ii) an optical interface that allows light to penetrate therethrough.