In vitro growth of tissues suitable to the formation of bone and bone forming tissue formed thereby
First Claim
1. A device for the growth of new tissue under in vitro cell culture conditions comprising a bioreactor, wherein the bioreactor comprisesan inlet port to receive nutrient solutions into the bioreactor,an outlet port to remove nutrient solutions from the bioreactor,sample injection ports,an inlet port and outlet port for the bioreactor to cyclically receive negative pressure and positive pressure,a bone particle or fiber,an outer nondeformable chamber and an inner deformable chamber, whereinthe inner deformable chamber comprises at least one deformable wall between the outer nondeformable chamber and the inner deformable chamber in at least two different dimensions,the at least one deformable wall comprises a flexible barrier, andthe flexible barrier is permeable to a metabolite having a molecular weight of less than 25 kiloDalton but is not permeable to metabolite having a molecular weight of 25 kiloDalton or more, andresorbable hollow fibers for the delivery of nutrients and removal of wastes, wherein the resorbed hollow fibers leave hollow tube-like openings in the new tissue, and wherein the bioreactor is capable of applying mechanical and electrical stimuli to the formed or forming new tissue.
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Accused Products
Abstract
The present invention is directed to a device for the growth of new bone or bone-like tissue under in vitro cell culture conditions.
36 Citations
48 Claims
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1. A device for the growth of new tissue under in vitro cell culture conditions comprising a bioreactor, wherein the bioreactor comprises
an inlet port to receive nutrient solutions into the bioreactor, an outlet port to remove nutrient solutions from the bioreactor, sample injection ports, an inlet port and outlet port for the bioreactor to cyclically receive negative pressure and positive pressure, a bone particle or fiber, an outer nondeformable chamber and an inner deformable chamber, wherein the inner deformable chamber comprises at least one deformable wall between the outer nondeformable chamber and the inner deformable chamber in at least two different dimensions, the at least one deformable wall comprises a flexible barrier, and the flexible barrier is permeable to a metabolite having a molecular weight of less than 25 kiloDalton but is not permeable to metabolite having a molecular weight of 25 kiloDalton or more, and resorbable hollow fibers for the delivery of nutrients and removal of wastes, wherein the resorbed hollow fibers leave hollow tube-like openings in the new tissue, and wherein the bioreactor is capable of applying mechanical and electrical stimuli to the formed or forming new tissue.
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47. A device for the growth of new tissue under in vitro cell culture conditions comprising a bioreactor, wherein the bioreactor comprises
an inlet port to receive nutrient solutions into the bioreactor, an outlet port to remove nutrient solutions from the bioreactor, sample injection ports, an inlet port and outlet port for the bioreactor to cyclically receive negative pressure and positive pressure, a bone particle or fiber, an outer nondeformable chamber and an inner deformable chamber, wherein there is a space(s) between the outer nondeformable chamber and the inner deformable chamber in at least two different dimensions, and resorbable hollow fibers for the delivery of nutrients and removal of wastes, wherein the resorbed hollow fibers leave hollow tube-like openings in the new tissue, and wherein the bioreactor is capable of applying mechanical and electrical stimuli to the formed or forming new tissue.
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48. A device for the growth of new tissue under in vitro cell culture conditions comprising a bioreactor, wherein the bioreactor comprises
an inlet port to receive nutrient solutions into the bioreactor, an outlet port to remove nutrient solutions from the bioreactor, sample injection ports, an inlet port and outlet port for the bioreactor to cyclically receive negative pressure and positive pressure, a bone particle or fiber, an outer nondeformable chamber and an inner deformable chamber, wherein the side wall(s) of the outer nondeformable chamber is distinct from the side wall(s) of the inner deformable chamber, and resorbable hollow fibers for the delivery of nutrients and removal of wastes, wherein the resorbed hollow fibers leave hollow tube-like openings in the new tissue, and wherein the bioreactor is capable of applying mechanical and electrical stimuli to the formed or forming new tissue.
Specification