Substrates containing polyphosphazene as matrices and substrates containing polyphosphazene with a micro-structured surface

• US 9,080,146 B2
• Filed: 09/25/2008
• Issued: 07/14/2015
• Est. Priority Date: 01/11/2001
• Status: Active Grant

• Current Assignee: Celonova Biosciences, Inc. (Boston Scientific Corporation)
• Original Assignee: Celonova Biosciences, Inc. (Boston Scientific Corporation)
• Inventors: Grunze, Michael, Fritz, Ulf
• Primary Examiner(s): Ware, Debbie K
• Application Number:
  US12/237,928
• Publication Number:
  US 20090117637A1
• Time in Patent Office: 2,483 Days
• US Class Current: 1/1
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/3687 characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: C08G 79/025 Polyphosphazenes
• CPC Class Codes: C08L 85/02 containing phosphorus
• CPC Class Codes: C09D 185/02 containing phosphorus
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 2535/10 Patterned coating
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

This disclosure relates to substrates containing at least one polyphosphazene with a forming surface as matrices for producing biological materials that can be implanted in a mammal. The disclosure also relates to a method for producing such substrates and substrates containing polyphosphazene with a micro-structured surface.

Claims

1. A substrate comprising a polyphosphazene micro-structured surface, wherein:

a) the polyphosphazene micro-structured substrate has a polyphosphazene film thickness from 65 nm to 290 nm;

b) the spacing density of the void structure within the polyphosphazene micro-structured substrate is from 650 voids per 100 μ

m² to 100 voids/100 μ

m²; and

c) the surface coverage of the polyphosphazene micro-structured substrate is from 92.5% to 97.5%, and

d) the polyphosphazene is present in the polyphosphazene film at a concentration from about 6 mg/mL to about 20 mg/mL,

wherein the substrate is selected from the group consisting of a polymer, a copolymer, a rubber material, an elastomer, an elastomeric membrane, a fabric, a polysaccharide, a glass, and combinations thereof, and

wherein the substrate exhibits cellular replusion.