Biosensor, biosensor chip and biosensor device

• US 9,080,954 B2
• Filed: 10/08/2014
• Issued: 07/14/2015
• Est. Priority Date: 07/02/2002
• Status: Expired due to Fees

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Inventors: Ueno, Hiroya, Nakatsuka, Junji
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US14/509,762
• Publication Number:
  US 20150053560A1
• Time in Patent Office: 279 Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/30 Electrodes, e.g. test electrodes; Half-cells G01N27/414 takes precedence
• CPC Class Codes: G01N 27/307 Disposable laminated or multilayered electrodes G01N27/3272 takes precedence
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3273 Devices therefor, e.g. test element readers, circuitry details not specific to biochemical electrodes G01N33/4875
• CPC Class Codes: G01N 27/416 Systems G01N27/27 takes precedence
• CPC Class Codes: G01R 19/0092 measuring current only all subgroups of G01R19/00 take precedence

Abstract

A biosensor includes a working electrode 101, a counter electrode 102 opposing the working electrode 101, a working electrode terminal 103 and a working electrode reference terminal 10 connected to the working electrode 101 by wires, and a counter electrode terminal 104 connected to the counter electrode 102 by a wire. By employing a structure with at least three electrodes, it is possible to assay a target substance without being influenced by the line resistance on the working electrode side.

Claims

1. A biosensor, comprising:

a working electrode;

a counter electrode;

a working electrode terminal;

a counter electrode terminal;

a first reference terminal;

a second reference terminal;

a first conductive line connecting the working electrode to the working electrode terminal;

a second conductive line connecting the working electrode to the first reference terminal, a portion of the first conductive line and the second conductive line is partially shared;

a third conductive line connecting the counter electrode to the counter electrode terminal; and

a fourth conductive line connecting the counter electrode to the second reference terminal.

2. The biosensor of claim 1, wherein the partially shared portion of the first conductive line and the second conductive line is connected to the working electrode.

3. The biosensor of claim 2, wherein:

the working electrode is disposed on a proximal end of the biosensor;

the working electrode terminal and the first reference terminal are disposed on a distal end of the biosensor; and

the partially shared portion of the first conductive line and the second conductive line branches into two lines.

4. The biosensor of claim 1, wherein:

the second conductive line is provided to enable a voltage at the working electrode to be detected; and

the fourth conductive line is provided to enable a voltage at the counter electrode to be detected.

5. The biosensor of claim 1, wherein the second conductive line and the fourth conductive line are provided to enable a voltage between the working electrode and the counter electrode to be constant.

6. The biosensor of claim 1, wherein at least a portion of the first conductive line is disposed between at least a portion of the second conductive line and at least a portion of the third conductive line on the biosensor.

7. The biosensor of claim 1, wherein the at least a portion of third conductive line is disposed between at least a portion of the first conductive line and at least a portion of the fourth conductive line on the biosensor.

8. The biosensor of claim 1, wherein:

a portion of the third conductive line and the fourth conductive line is partially shared; and

the partially shared portion of the third conductive line and the fourth conductive line is connected to the counter electrode.

9. The biosensor of claim 1, further comprising an additional counter electrode disposed on the proximal end of the biosensor.

10. The biosensor of claim 9, wherein the working electrode is provided between the counter electrode and the additional counter electrode.

11. The biosensor of claim 9, wherein the counter electrode and the additional counter electrode share the working electrode.

12. The biosensor of claim 1, wherein the first reference terminal is disposed near an outside edge of the biosensor relative to a centerline that extends between a proximal end and a distal end of the biosensor.

13. The biosensor of claim 1, wherein an inner edge of the first reference terminal is disposed outside an inner edge of the working electrode terminal relative to a centerline that extends between a proximal end and a distal end of the biosensor.

14. The biosensor of claim 1, wherein the second reference terminal is disposed near an outside edge of the biosensor relative to a centerline that extends between a proximal end and a distal end of the biosensor.

15. The biosensor of claim 1, wherein an inner edge of the second reference terminal is disposed outside an inner edge of the counter electrode terminal relative to a centerline that extends between a proximal end and a distal end of the biosensor.

16. The biosensor of claim 1, further comprising an assay reagent applied on the working electrode and on the counter electrode.

17. A biosensor device, comprising:

a biosensor, comprising;

a working electrode;

a counter electrode;

a working electrode terminal;

a counter electrode terminal;

a first reference terminal;

a second reference terminal;

a first conductive line connecting the working electrode to the working electrode terminal;

a second conductive line connecting the working electrode to the first reference terminal, a portion of the second conductive line and the first conductive line is partially shared;

a third conductive line connecting the counter electrode to the counter electrode terminal; and

a fourth conductive line connecting the counter electrode to the second reference terminal; and

a measurement circuit configured to be connected to the biosensor for performing an assay of a fluid to determine the concentration of a substance in the fluid based on a current flowing between the working electrode and the counter electrode;

wherein substantially no current flows through the second conductive line and the fourth conductive line during the assay.

18. The biosensor device of claim 17, wherein the partially shared portion of the first conductive line and the second conductive line is connected to the working electrode.

19. The biosensor device of claim 18, wherein:

the working electrode is disposed on a proximal end of the biosensor;

the working electrode terminal and the first reference terminal are disposed on a distal end of the biosensor; and

the partially shared portion of the first conductive line and the second conductive line branches into two lines.

20. The biosensor device of claim 17, wherein at least a portion of the first conductive line is disposed between the at least a portion of second conductive line and at least a portion of the third conductive line on the biosensor.

21. The biosensor device of claim 17, wherein at least a portion of the third conductive line is disposed between at least a portion of the first conductive line and at least a portion of the fourth conductive line on the biosensor.

22. The biosensor device of claim 17, wherein:

a portion of the third conductive line and the fourth conductive line is partially shared; and

the partially shared portion of the third conductive line and the fourth conductive line is connected to the counter electrode.

23. The biosensor device of claim 17, further comprising an additional counter electrode disposed on the proximal end of the biosensor.

24. The biosensor of claim 23, wherein the working electrode is provided between the counter electrode and the additional counter electrode.

25. The biosensor of claim 23, wherein the counter electrode and the additional counter electrode share the working electrode.

26. The biosensor device of claim 17, wherein the first reference terminal is disposed near an outside edge of the biosensor relative to a centerline that extends between a proximal end and a distal end of the biosensor.

27. The biosensor device of claim 17, wherein the second reference terminal is disposed near an outside edge of the biosensor relative to a centerline that extends between a proximal end and a distal end of the biosensor.

28. The biosensor device of claim 17, further comprising an assay reagent applied on the working electrode and on the counter electrode.