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Production of motif-specific and context-independent antibodies using peptide libraries as antigens

  • US 9,085,609 B2
  • Filed: 07/11/2006
  • Issued: 07/21/2015
  • Est. Priority Date: 09/04/1998
  • Status: Active Grant
First Claim
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1. A purified motif-specific, context-independent antibody that specifically binds a motif consisting of (i) two to six invariant amino acids including at least one methylated amino acid, and, optionally, (ii) one or more degenerate amino acid position(s), wherein said motif is selected from the group consisting of monomethylarginine-G-G, monomethylarginine-G-G-monomethylarginine-G-G (SEQ ID NO:

  • 311), monomethylarginine-X-monomethylarginine, monomethylarginine-G, and G-monomethylarginine-G and wherein said antibody specifically binds said motif in a plurality of non-homologous peptides or proteins within an organism.

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