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Isolation, cultivation and uses of stem/progenitor cells

  • US 9,085,755 B2
  • Filed: 02/28/2013
  • Issued: 07/21/2015
  • Est. Priority Date: 08/16/2004
  • Status: Active Grant
First Claim
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1. A method for isolating stem/progenitor cells from the amniotic membrane of an umbilical cord, the method comprising:

  • (a) separating the amniotic membrane from the other components of the umbilical cord in vitro to obtain an amniotic membrane tissue;

    (b) culturing the amniotic membrane tissue obtained in step (a) under conditions allowing cell proliferation; and

    (c) isolating the stem/progenitor cells, wherein the stem/progenitor cells are epithelial or mensenchymal stem/progenitor cells expressing the following genes;

    POU5fl, Bmi-1, leukemia inhibitory factor (LIF), and secreting Activin A and follistatin.

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