Methods for identifying nucleic acid ligands
First Claim
1. A method for identifying a nucleic acid ligand of a target molecule from a candidate mixture of nucleic acids, the method comprising:
- contacting at least one target molecule with a solution comprising a candidate mixture of nucleic acids, wherein the nucleic acids have different affinities for the target molecule; and
separating in a single step nucleic acids that bind the target molecule with greatest affinity from nucleic acids that bind the target molecule with a lesser affinity and nucleic acids that do not bind the target molecule, wherein separating is by a liquid chromatography or gel technique using a gradient profile that comprises a linearly increasing concentration of the target molecule and the end portion of the gradient profile comprises a linearly increasing concentration of the target molecule and a linearly increasing concentration of a chaotropic agent, and wherein the separating step is performed only a single time, thereby identifying the nucleic acid ligand to the target molecule.
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Abstract
The present invention generally relates to methods for identifying nucleic acid ligands of a target molecule. In certain embodiments, the invention provides methods for identifying a nucleic acid ligand of a target molecule from a candidate mixture of nucleic acids, including contacting at least one target molecule with a candidate mixture of nucleic acids, in which the nucleic acids have different affinities for the target molecule, and separating in a single step nucleic acids that bind the target molecule with greatest affinity from nucleic acids that bind the target molecule with a lesser affinity and nucleic acids that do not bind the target molecule, thereby identifying the nucleic acid ligand of the target molecule.
299 Citations
7 Claims
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1. A method for identifying a nucleic acid ligand of a target molecule from a candidate mixture of nucleic acids, the method comprising:
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contacting at least one target molecule with a solution comprising a candidate mixture of nucleic acids, wherein the nucleic acids have different affinities for the target molecule; and separating in a single step nucleic acids that bind the target molecule with greatest affinity from nucleic acids that bind the target molecule with a lesser affinity and nucleic acids that do not bind the target molecule, wherein separating is by a liquid chromatography or gel technique using a gradient profile that comprises a linearly increasing concentration of the target molecule and the end portion of the gradient profile comprises a linearly increasing concentration of the target molecule and a linearly increasing concentration of a chaotropic agent, and wherein the separating step is performed only a single time, thereby identifying the nucleic acid ligand to the target molecule. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification