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Methods for identifying nucleic acid ligands

  • US 9,085,773 B2
  • Filed: 05/07/2013
  • Issued: 07/21/2015
  • Est. Priority Date: 11/03/2009
  • Status: Active Grant
First Claim
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1. A method for identifying a nucleic acid ligand of a target molecule from a candidate mixture of nucleic acids, the method comprising:

  • contacting at least one target molecule with a solution comprising a candidate mixture of nucleic acids, wherein the nucleic acids have different affinities for the target molecule; and

    separating in a single step nucleic acids that bind the target molecule with greatest affinity from nucleic acids that bind the target molecule with a lesser affinity and nucleic acids that do not bind the target molecule, wherein separating is by a liquid chromatography or gel technique using a gradient profile that comprises a linearly increasing concentration of the target molecule and the end portion of the gradient profile comprises a linearly increasing concentration of the target molecule and a linearly increasing concentration of a chaotropic agent, and wherein the separating step is performed only a single time, thereby identifying the nucleic acid ligand to the target molecule.

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