Systems and methods for detection and quantification of analytes
First Claim
1. A method for identifying a presence and/or quantity of a target analyte within a sample in a cartridge, the method comprising:
- initiating a mixing protocol to form a mixture within a reservoir of the cartridge, wherein the mixture comprises a plurality of magnetic particles each bound directly or indirectly to a respective one of a plurality of agents comprising oxidizing enzymes and/or the target analyte present in the sample;
facilitating movement of the mixture from the reservoir into an analysis channel of the cartridge, wherein the magnetic particles and the agents and/or the target analyte bound thereto localize on a portion of the analysis channel within a magnetic field, forming a localized sample;
facilitating movement of a plurality of substrates into the analysis channel, wherein the oxidizing enzymes of the agents within the localized sample oxidize the substrates;
receiving a signal from an electrochemical sensor, wherein at least a portion of the signal results from the oxidation of the substrates; and
analyzing the signal to identify the presence and/or quantity of the target analyte in the sample.
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Accused Products
Abstract
Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.
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Citations
20 Claims
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1. A method for identifying a presence and/or quantity of a target analyte within a sample in a cartridge, the method comprising:
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initiating a mixing protocol to form a mixture within a reservoir of the cartridge, wherein the mixture comprises a plurality of magnetic particles each bound directly or indirectly to a respective one of a plurality of agents comprising oxidizing enzymes and/or the target analyte present in the sample; facilitating movement of the mixture from the reservoir into an analysis channel of the cartridge, wherein the magnetic particles and the agents and/or the target analyte bound thereto localize on a portion of the analysis channel within a magnetic field, forming a localized sample; facilitating movement of a plurality of substrates into the analysis channel, wherein the oxidizing enzymes of the agents within the localized sample oxidize the substrates; receiving a signal from an electrochemical sensor, wherein at least a portion of the signal results from the oxidation of the substrates; and analyzing the signal to identify the presence and/or quantity of the target analyte in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification