Method for determining equivalent thermal conditions between liquid chromatography systems
First Claim
1. A method of transferring a chromatographic method between liquid chromatography (LC) systems, the method comprising:
- determining an isoretention temperature of a first LC system at which a first solute and a second solute in a mobile phase of the first LC system co-elute from a column;
determining an isoretention temperature of a second LC system at which the first solute and the second solute in a mobile phase of the second LC system co-elute from a column; and
determining a column offset temperature for transferring a chromatographic method between the first and second LC systems based on the difference in the isoretention temperatures of the first and second LC systems.
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Abstract
Described is a method of transferring a chromatographic method between liquid chromatography (LC) systems. The method is based on a determination of an isoretention temperature at which two solutes co-elute. The method enables separations to be performed using different LC systems with reproducible and equivalent results. For example, the method allows for a chromatography method developed for HPLC to be more readily transferred to a UPLC system and for a chromatography method developed for a UPLC system to be more readily transferred to a HPLC system. The method addresses LC systems having column ovens of different design in which the internal column temperatures are not equal although the operating temperatures of the column ovens may be accurately controlled to equal values. The retention behavior and resolution of different LC systems is caused to be substantially the same so that equivalent separation results are obtained.
9 Citations
19 Claims
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1. A method of transferring a chromatographic method between liquid chromatography (LC) systems, the method comprising:
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determining an isoretention temperature of a first LC system at which a first solute and a second solute in a mobile phase of the first LC system co-elute from a column; determining an isoretention temperature of a second LC system at which the first solute and the second solute in a mobile phase of the second LC system co-elute from a column; and determining a column offset temperature for transferring a chromatographic method between the first and second LC systems based on the difference in the isoretention temperatures of the first and second LC systems. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of transferring a chromatographic method between liquid chromatography (LC) systems, the method comprising:
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determining, for a plurality of solute pairs each having a first solute and a second solute, an isoretention temperature of a first LC system at which the first solute and the second solute in a mobile phase of the first LC system co-elute from a column; determining, for each of the solute pairs, an isoretention temperature of a second LC system at which the first solute and the second solute in a mobile phase of the second LC system co-elute from a column; and determining, for each of the solute pairs, a column offset temperature for transferring a chromatographic method between the first and second LC systems based on the difference in a respective pair of isoretention temperatures of the first and second LC systems. - View Dependent Claims (11, 12, 13)
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14. A method of transferring a chromatographic method between liquid chromatography (LC) systems, the method comprising:
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determining a van'"'"'t Hoff functional relationship for a first solute in a mobile phase for a plurality of column temperatures for a first LC system; determining a van'"'"'t Hoff functional relationship for a second solute in a mobile phase for a plurality of column temperatures for the first LC system; determining an isoretention temperature of the first LC system for the first and second solutes as a temperature at which a retention factor of each of the van'"'"'t Hoff functional relationships of the two solutes in the first LC system are equal; determining a van'"'"'t Hoff functional relationship for the first solute in a mobile phase for a plurality of column temperatures for a second LC system; determining a van'"'"'t Hoff functional relationship for the second solute in a mobile phase for a plurality of column temperatures for the second LC system; determining an isoretention temperature of the second LC system for the first and second solutes as a temperature at which a retention factor of each of the van'"'"'t Hoff functional relationships of the two solutes in the second LC system are equal; and determining a column offset temperature for transferring a chromatographic method between the first and second LC systems based on the difference in the isoretention temperatures of the first and second LC systems. - View Dependent Claims (15, 16)
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17. A method of transferring a chromatographic method between liquid chromatography (LC) systems, the method comprising:
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determining, for a plurality of first solutes, a van'"'"'t Hoff functional relationship for the first solute in a mobile phase for a plurality of column temperatures for a first LC system; determining, for a plurality of second solutes, a van'"'"'t Hoff functional relationship for the second solute in a mobile phase for a plurality of column temperatures for the first LC system; determining, for a plurality of solute pairs each comprising one of the first solutes and one of the second solutes, an isoretention temperature of the first LC system as a temperature at which a retention factor of the van'"'"'t Hoff functional relationships of the first and second solutes in the first LC system are equal; determining, for the plurality of first solutes, a van'"'"'t Hoff functional relationship for the first solute in a mobile phase for a plurality of column temperatures for a second LC system; determining, for the plurality of second solutes, a van'"'"'t Hoff functional relationship for the second solute in a mobile phase for a plurality of column temperatures for the second LC system; determining, for the plurality of solute pairs, an isoretention temperature of the second LC system as a temperature at which the retention factor of the van'"'"'t Hoff functional relationships of the first and second solutes in the second LC system are equal; and determining, for each of the solute pairs, a column offset temperature for transferring a chromatographic method between the first and second LC systems based on a difference in a respective pair of isoretention temperatures of the first and second LC systems. - View Dependent Claims (18, 19)
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Specification