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A-β immunogenic peptide carrier conjugates and methods of producing same

  • US 9,089,510 B2
  • Filed: 07/07/2011
  • Issued: 07/28/2015
  • Est. Priority Date: 12/17/2003
  • Status: Expired due to Fees
First Claim
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1. A method of producing an immunogenic conjugate, the method comprising the steps of:

  • (a) derivatizing one or more functional groups of a carrier protein to generate an activated functional group on the carrier protein;

    (b) reacting a peptide immunogen comprising an Aβ

    fragment with the carrier protein of step (a) under conditions to form a conjugate, wherein the Aβ

    fragment comprises residues 16-23 of Aβ

    (SEQ ID NO;

    21) and the peptide immunogen is covalently attached to the activated functional group on the carrier protein; and

    (c) further reacting the conjugate of step (b) with N-acetylcysteamine to inactivate any remaining activated functional group on the carrier protein to generate the immunogenic conjugate, wherein a capping molecule, formed by reaction of the conjugate with the N-acetylcysteamine, is covalently attached to the remaining activated functional group on the carrier protein,wherein the immunogenic conjugate has the formula;

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