Multi-modal surface plasmon polariton—raman scattering based bio-detection

• US 9,089,842 B2
• Filed: 04/29/2010
• Issued: 07/28/2015
• Est. Priority Date: 03/12/2008
• Status: Active Grant

• Current Assignee: Mitre Corporation
• Original Assignee: Mitre Corporation
• Inventors: Hwang, Grace M., Pang, Lin, Fainman, Yeshaiahu
• Primary Examiner(s): Gross, Christopher M
• Application Number:
  US12/770,411
• Publication Number:
  US 20100208253A1
• Time in Patent Office: 1,916 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/554 detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons

Abstract

Methods and systems for combined SPP and Raman scattering-based bio-detection are provided. Embodiments include a bio-detection system having a microfluidics chip, a Surface Plasmon Polariton (SPP)-based system component, and a Raman scattering-based system component. The SPP-based and the Raman scattering-based system components can be used simultaneously or individually separately to detect biological and/or chemical analytes. The bio-detection system further includes an aerosol collector chip. Embodiments of the present invention can be used aboard means of propagation of biological and/or chemical analytes, including, for example, commercial aircrafts. Embodiments of the present invention can be used to enable an aircraft warning system.

Claims

1. A method for bio-detection using a microfluidics chip having a liquid-metal (LM) interface and a substrate-metal (SM) interface, comprising:

directing light at said microfluidics chip;

measuring a first energy associated with photons of said light that interact with a first surface plasmon polariton (SPP) mode of said LM interface;

measuring changes in said first energy;

measuring a second energy associated with photons of said light that interact with a second SPP mode of said SM interface;

measuring changes in said second energy;

calibrating said changes in said first energy according to said changes in said second energy; and

detecting, using said calibrated changes in said first energy, refractive index changes within said microfluidics chip, wherein said refractive index changes result from bio-receptor molecules within said microfluidics chip binding with analytes,

wherein said microfluidics chip comprises a nanohole array.

2. The method of claim 1, wherein said light includes a laser beam.

3. The method of claim 2, further comprising:

controlling said laser beam to generate said SPP mode along said LM interface of said microfluidics chip and said second SPP mode along said SM interface of said microfluidics chip.

4. The method of claim 1,

wherein said changes in said first energy occur as a result of said refractive index changes within said microfluidics chip.

5. The method of claim 1, wherein said first SPP mode varies when binding occurs between said bio-receptor molecules and said analytes, and wherein said second SPP mode is invariant to said binding.

6. The method of claim 1, further comprising:

detecting Raman scattered photons that result from changes in vibrational, rotational, or electronic energy of said bio-receptor molecules when said bio-receptor molecules bind with said analytes.

7. The method of claim 6, wherein said step of detecting Raman scattered photons comprises:

generating a wavelength spectrum of photons scattered as a result of said light being directed at said microfluidics chip; and

detecting scattered photons having wavelengths different than a wavelength of said light.

8. The method of claim 6, further comprising:

combining detection results from said detecting steps to generate a bio-detection result, wherein said bio-detection result indicates a presence of said analytes or a lack thereof.

9. The method of claim 1, wherein said bio-receptor molecules include one or more of complex carbohydrates, lectins, peptides, or anti-bodies.

10. The method of claim 9, wherein said bio-receptor molecules are applied simultaneously to different areas of a gold layer of said microfluidics chip, and wherein each of said bio-receptor molecules is dedicated to detecting a respective analyte, thereby allowing multi-element bio-detection.

12. The method of claim 1, wherein said changes in said second energy are due to variations in temperature, pressure, and/or flow.

13. The method of claim 12, wherein said calibrated changes in said first energy are substantially free of changes due to variations in temperature, pressure, and/or flow.

11. A method for bio-detection using a microfluidics chip having a liquid-metal (LM) interface and a substrate-metal (SM) interface, comprising:

measuring a first energy associated with photons of light that interact with a first surface plasmon polariton (SPP) mode of said LM interface of said microfluidics chip;

measuring changes in said first energy;

measuring a second energy associated with photons of light that interact with a second SPP mode of said SM interface of said microfluidics chip;

measuring changes in said second energy;

calibrating said changes in said first energy according to said changes in said second energy; and

detecting, using said calibrated changes in said first energy, refractive index changes within said microfluidics chip, wherein said refractive index changes result from bio-receptor molecules within said microfluidics chip binding with analytes,

wherein said microfluidics chip comprises a nanohole array.

14. The method of claim 11, further comprising:

detecting Raman scattered photons that result from changes in vibrational, rotational, or electronic energy of said bio-receptor molecules when said bio-receptor molecules bind with said analytes.

15. The method of claim 14, further comprising:

combining detection results from said detecting steps to generate a bio-detection result, wherein said bio-detection result indicates a presence of said analytes or a lack thereof.

16. The method of claim 11, wherein said first SPP mode varies when binding occurs between said bio-receptor molecules and said analytes, and wherein said second SPP mode is invariant to said binding.

17. The method of claim 11, further comprising:

directing light at said LM interface of said microfluidics chip to generate said first SPP mode; and

directing light at said SM interface of said microfluidics chip to generate said second SPP mode.

18. The method of claim 11, wherein said changes in said second energy are due to variations in temperature, pressure, and/or flow.

19. The method of claim 18, wherein said calibrated changes in said first energy are substantially free of changes due to variations in temperature, pressure, and/or flow.