Antibody targeting osteoclast-related protein Siglec-15
First Claim
1. A method of treating abnormal bone metabolism comprising administering a pharmaceutical composition comprising a monoclonal antibody or an antigen binding fragment thereof to a subject in need thereof, wherein the monoclonal antibody or the antigen binding fragment of the monoclonal antibody directly binds one or more amino acid sequences selected from the group consisting of:
- (a) amino acid residues 21 to 328 of the amino acid sequence of SEQ ID NO;
2;
(b) amino acid residues 1 to 260 of the amino acid sequence of SEQ ID NO;
2;
(c) amino acid residues 21 to 260 of the amino acid sequence of SEQ ID NO;
2;
(d) amino acid residues 21 to 341 of the amino acid sequence of SEQ ID NO;
4;
(e) amino acid residues 1 to 258 of the amino acid sequence of SEQ ID NO;
4; and
(f) amino acid residues 21 to 258 of the amino acid sequence of SEQ ID NO;
4,where (a)-(f) are in one or more polypeptides;
wherein the monoclonal antibody or the antigen binding fragment of the monoclonal antibody contains the same Complementarity Determining Regions (CDRs) as a monoclonal antibody produced by a hybridoma selected from the group consisting of hybridoma #32A1 (FERM BP-10999) and hybridoma #41B1 (FERM BP-11000);
wherein said binding of said antibody or antigen binding fragment inhibits osteoclast formation and/or osteoclastic bone resorption; and
wherein the abnormal bone metabolism is characterized by insufficient bone growth, mass, or density.
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Abstract
To provide a method of detecting abnormal bone metabolism by using a gene strongly expressed in an osteoclast; a method of screening a compound having a therapeutic and/or preventive effect on abnormal bone metabolism; and a pharmaceutical composition for treating and/or preventing abnormal bone metabolism. Provision of a method of detecting abnormal bone metabolism by using the expression of human Siglec-15 gene as an index; a pharmaceutical composition containing an antibody which specifically recognizes human Siglec-15 and has an activity of inhibiting osteoclast formation; and the like.
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Citations
26 Claims
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1. A method of treating abnormal bone metabolism comprising administering a pharmaceutical composition comprising a monoclonal antibody or an antigen binding fragment thereof to a subject in need thereof, wherein the monoclonal antibody or the antigen binding fragment of the monoclonal antibody directly binds one or more amino acid sequences selected from the group consisting of:
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(a) amino acid residues 21 to 328 of the amino acid sequence of SEQ ID NO;
2;(b) amino acid residues 1 to 260 of the amino acid sequence of SEQ ID NO;
2;(c) amino acid residues 21 to 260 of the amino acid sequence of SEQ ID NO;
2;(d) amino acid residues 21 to 341 of the amino acid sequence of SEQ ID NO;
4;(e) amino acid residues 1 to 258 of the amino acid sequence of SEQ ID NO;
4; and(f) amino acid residues 21 to 258 of the amino acid sequence of SEQ ID NO;
4,where (a)-(f) are in one or more polypeptides; wherein the monoclonal antibody or the antigen binding fragment of the monoclonal antibody contains the same Complementarity Determining Regions (CDRs) as a monoclonal antibody produced by a hybridoma selected from the group consisting of hybridoma #32A1 (FERM BP-10999) and hybridoma #41B1 (FERM BP-11000); wherein said binding of said antibody or antigen binding fragment inhibits osteoclast formation and/or osteoclastic bone resorption; and wherein the abnormal bone metabolism is characterized by insufficient bone growth, mass, or density. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 25, 26)
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23. A method of treating abnormal bone metabolism comprising administering a pharmaceutical composition comprising a monoclonal antibody or an antigen binding fragment thereof to a subject in need thereof;
- wherein the monoclonal antibody or the antigen binding fragment of the monoclonal antibody is produced by a hybridoma selected from the group consisting of hybridoma #32A1 (FERM BP-10999) and hybridoma #41B1 (FERM BP-11000); and
wherein the abnormal bone metabolism is characterized by insufficient bone growth, mass, or density.
- wherein the monoclonal antibody or the antigen binding fragment of the monoclonal antibody is produced by a hybridoma selected from the group consisting of hybridoma #32A1 (FERM BP-10999) and hybridoma #41B1 (FERM BP-11000); and
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24. A method of treating abnormal bone metabolism comprising administering a pharmaceutical composition comprising a monoclonal antibody or an antigen binding fragment thereof to a subject in need thereof;
- wherein the monoclonal antibody or the antigen binding fragment of the monoclonal antibody directly binds one or more amino acid sequences encoded by a nucleotide sequence selected from the group consisting of;
(a) the nucleotide sequence of SEQ ID NO;
19;(b) the nucleotide sequence of SEQ ID NO;
43;(c) the nucleotide sequence of SEQ ID NO;
1; and(d) the nucleotide sequence of SEQ ID NO;
3,where (a)-(d) are in one or more polypeptides; wherein the monoclonal antibody or the antigen binding fragment of the monoclonal antibody contains the same Complementarity Determining Regions (CDRs) as a monoclonal antibody produced by a hybridoma selected from the group consisting of hybridoma #32A1 (FERM BP-10999) and hybridoma #41B1 (FERM BP-11000); wherein said binding of said antibody or antigen binding fragment inhibits osteoclast formation and/or osteoclastic bone resorption; and wherein the abnormal bone metabolism is characterized by insufficient bone growth, mass, or density.
- wherein the monoclonal antibody or the antigen binding fragment of the monoclonal antibody directly binds one or more amino acid sequences encoded by a nucleotide sequence selected from the group consisting of;
Specification