Method for measuring glycated hemoglobin

• US 9,090,931 B2
• Filed: 08/09/2011
• Issued: 07/28/2015
• Est. Priority Date: 08/11/2010
• Status: Active Grant

• Current Assignee: Kyowa Medex Co., Ltd. (Resonac Holdings Corp.)
• Original Assignee: Kyowa Medex Co., Ltd. (Resonac Holdings Corp.)
• Inventors: Soya, Haruyo, Murakami, Tomomi, Tsunoda, Haruki, Ohsugi, Yu, Yoda, Ayako, Matsushita, Masashi
• Primary Examiner(s): McDonald, Jennifer
• Assistant Examiner(s): Xu, Qing
• Application Number:
  US13/811,914
• Publication Number:
  US 20130115646A1
• Time in Patent Office: 1,449 Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 1/37 involving peptidase or proteinase
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 33/723 Glycosylated haemoglobin

Abstract

It is to provide a method for measuring glycated hemoglobin in a hemoglobin-containing sample, comprising: reacting glycated hemoglobin in the hemoglobin-containing sample with a proteolytic enzyme in the presence of at least one salt selected from the group consisting of a pyridinium salt, a phosphonium salt, an imidazolium salt, and an isoquinolinium salt; reacting the obtained reaction product with fructosyl peptide oxidase; and measuring the generated hydrogen peroxide. The present invention provides a method for accurately measuring glycated hemoglobin in a hemoglobin-containing sample.

Claims

1. A method for measuring glycated hemoglobin in a hemoglobin-containing sample, comprising:

reacting the glycated hemoglobin in the hemoglobin-containing sample with a proteolytic enzyme in the presence of a pyridinium salt represented by the following formula (I) to obtain a reaction product comprising a glycated peptide;

reacting the obtained reaction product with fructosyl peptide oxidase to generate hydrogen peroxide; and

measuring the generated hydrogen peroxide;

2. The method according to claim 1, wherein the measuring hydrogen peroxide is performed by adding a hydrogen peroxide measuring agent to the generated hydrogen peroxide.

3. The method according to claim 2, wherein the hydrogen peroxide measuring reagent is a reagent comprising peroxidase and a leuco chromogen.

4. The method according to claim 3, wherein the leuco chromogen is a phenothiazine chromogen.

5. The method according to claim 4, wherein the phenothiazine chromogen is 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino)phenothiazine.