Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same

• US 9,096,899 B2
• Filed: 10/20/2011
• Issued: 08/04/2015
• Est. Priority Date: 10/27/2010
• Status: Active Grant

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Eltoukhy, Helmy A., Khurana, Tarun, Javanmardi, Behnam, Sabounchi, Poorya, Aghababazadeh, Majid
• Primary Examiner(s): Sines, Brian J
• Application Number:
  US13/882,088
• Publication Number:
  US 20130210682A1
• Time in Patent Office: 1,384 Days
• Field of Search: 422/50, 422/68.1, 422/502, 422/503, 422/547, 422/554, 422/82.05, 436/43
• US Class Current: 1/1
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/058 Flat flow cell
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/11 Filling or emptying of cuvettes
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 35/1097 characterised by the valves valves in general F16K

Abstract

Biosensor cartridge includes a flow cell having inlet and outlet ports and a flow channel that extends therebetween. The flow cell includes a substrate field having a plurality of reaction chambers. The reaction chambers have apertures that open onto the flow channel. The biosensor cartridge also includes an activity detector that is coupled to the flow cell and has an array of pixels that has a fixed position relative to the substrate field of the flow cell. The pixels are assigned to select reaction chambers such that activity detected by the pixels indicates that a desired reaction has occurred within the select reaction chamber. The biosensor cartridge also includes an exterior side surface having a plurality of electrical contacts thereon that are communicatively coupled to the pixels. The electrical contacts of the side surface are configured to engage corresponding mating contacts of a bioassay system.

Claims

1. A biosensor cartridge comprising:

a flow cell including inlet and outlet ports and a flow channel that extends therebetween, the flow cell including a substrate field comprising a plurality of reaction chambers, the reaction chambers having apertures that open onto the flow channel such that the reaction chambers are in fluid communication with the flow channel;

an activity detector coupled to the flow cell and including an array of pixels that has a fixed position relative to the substrate field of the flow cell, the pixels being assigned to select reaction chambers such that activity detected by the pixels indicates that a desired reaction has occurred within the select reaction chamber; and

an exterior side surface having a plurality of electrical contacts thereon that are communicatively coupled to the pixels, the electrical contacts of the side surface being configured to engage corresponding mating contacts of a bioassay system.

2. The biosensor cartridge of claim 1 wherein the electrical contacts comprise contact pads configured to engage the corresponding mating contacts of the bioassay system.

3. The biosensor cartridge of claim 1 wherein the side surface is a first side surface and the biosensor cartridge further comprises a second side surface, the second side surface including respective openings to the inlet and outlet ports, the openings being configured to removably engage nozzles of the bioassay system to fluidicly couple the flow channel to the bioassay system.

4. The biosensor cartridge of claim 3 wherein the first side surface faces in a mating direction, the second side surface receiving the nozzles through the corresponding openings of the inlet and outlet ports when the nozzles approach the second side surface in the mating direction, wherein the electrical contacts of the first side surface are pressed against corresponding mating contacts of the bioassay system when the nozzles engage the corresponding inlet and outlet ports.

5. The biosensor cartridge of claim 1 wherein the side surface is a first side surface and the biosensor cartridge further comprises a second side surface, the second side surface including an engagement area that extends along and proximate to the flow channel, the engagement area being sized and shaped to interface with a thermal element of the bioassay system.

6. The biosensor cartridge of claim 4 wherein the first side surface faces in a mating direction, the second side surface receiving the thermal element when the thermal element approaches the engagement area in the mating direction, wherein the electrical contacts of the first side surface are pressed against corresponding mating contacts of the bioassay system when the thermal element interfaces with the engagement area.

7. The biosensor cartridge of claim 1 wherein the side surface is a first side surface and the biosensor cartridge further comprises a second side surface, the first and second surfaces facing in opposite directions, wherein the second side surface is configured to engage a mating face of the bioassay system to facilitate maintaining an electrical connection between the electrical contacts of the first side surface and corresponding mating contacts of the bioassay system.

8. The biosensor cartridge of claim 1 wherein the flow channel includes inlet and outlet channel portions that extend from the respective openings of the inlet and outlet ports and a field charnel portion that extends along the substrate field, wherein the inlet and outlet channel portions extend parallel to a port plane and the field channel portion extends parallel to a channel plane, the channel and port planes being substantially perpendicular to one another.

9. The biosensor cartridge of claim 1 farther comprising an identification component configured to provide identification information to the bioassay system, the identification information relating to the biosensor cartridge.

10. The biosensor cartridge of claim 9 wherein the identification component comprises an RFID tag.

11. The biosensor cartridge of claim 9 wherein the identification component communicates to the bioassay system through the electrical contacts.

12. The biosensor cartridge of claim 1 wherein the flow cell includes a substrate layer that includes the substrate field and the plurality of reaction chambers, the substrate layer having a thickness that extends between the flow channel and the activity detector.

13. The biosensor cartridge of claim 12, wherein the substrate layer comprises a fiber-optic faceplate, the reaction chambers being formed by recesses in the faceplate.

14. The biosensor cartridge of claim 12, wherein the flow cell includes a flow cover that is mounted over the substrate layer.

15. The biosensor cartridge of claim 14, wherein the flow cover includes a cover wall that extends over the substrate field and comprises a substantially uniform thickness of thermally conductive material.

16. The biosensor cartridge of claim 1, wherein the activity detector comprises circuitry that receives and automatically associates detection signals provided by the pixels with the select reaction chambers, the detection signals being representative of light signals detected by the corresponding pixels.

17. The biosensor cartridge of claim 1, wherein the activity detector comprises circuitry that receives detection signals from the pixels that are representative of light signals detected by the pixels, the circuitry configured to analyze the detection signals and generate detection data based on the detection signals by processing the detection signals in a designated manner.

18. The biosensor cartridge of claim 1, wherein the activity detector comprises circuitry that receives detection signals from the pixels that are representative of light signals detected by the pixels, the circuitry configured to automatically modify values for designated pixels, the values being determined by the detection signals of the designated pixels.

19. The biosensor cartridge of claim 1, wherein the biosensor cartridge is a self-contained, disposable unit.

20. The biosensor cartridge of claim 1, wherein the flow cell and activity detector are coupled together to form a unitary structure.