Electrochemical detection of beta-lactoglobulin

• US 9,097,653 B2
• Filed: 08/07/2014
• Issued: 08/04/2015
• Est. Priority Date: 03/15/2013
• Status: Active Grant

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Gunasekaran, Sundaram, Yang, Jiang
• Primary Examiner(s): DIETERLE, JENNIFER M
• Application Number:
  US14/453,964
• Publication Number:
  US 20140346060A1
• Time in Patent Office: 362 Days
• Field of Search: 205/775, 205/777.5, 422/74
• US Class Current: 1/1
• CPC Class Codes: G01N 27/26 by investigating electrochemical variables; by using electrolysis or electrophoresis
• CPC Class Codes: G01N 27/28 Electrolytic cell components
• CPC Class Codes: G01N 27/30 Electrodes, e.g. test electrodes; Half-cells G01N27/414 takes precedence

Abstract

A method to detect beta-lactoglobulin (BLG) is described. The method includes the steps of adding a known concentration of hydrogen peroxide to a sample known to, or suspected of containing BLG; and electrolyzing the sample using a working electrode at a fixed potential sufficient to electrolyze BLG, and measuring a current signal within the sample. A diminution of the current signal in the sample as compared to a corresponding current signal from a standard solution containing a known concentration of hydrogen peroxide and no BLG indicates that the sample contains BLG.

Claims

1. A method to detect beta-lactoglobulin (BLG), the method comprising:

(a) adding hydrogen peroxide to a sample known to, or suspected of containing BLG; and

(b) electrolyzing the sample of step (a) using a working electrode at a potential sufficient to electrolyze BLG, and measuring a current signal within the sample, wherein a diminution of the current signal in the sample as compared to a corresponding current signal from a standard solution containing hydrogen peroxide and no BLG indicates that the sample contains BLG.

2. The method of claim 1, wherein step (b) comprises electrolyzing the sample using a working electrode comprising a transition metal or an oxide of a transition metal.

3. The method of claim 1, wherein step (b) comprises electrolyzing the sample using a working electrode comprising a transition metal.

4. The method of claim 1, wherein step (b) comprises electrolyzing the sample using a working electrode comprising an oxide of a transition metal.

5. The method of claim 1, wherein step (b) comprises electrolyzing the sample using a working electrode comprising an element selected from the group consisting of ruthenium, rhodium, palladium, platinum, silver, osmium, iridium, gold, mercury, rhenium, titanium, niobium, tantalum, or any combination thereof.

6. The method of claim 1, wherein step (b) comprises electrolyzing the sample using a working electrode comprising a metal oxide selected from the group consisting of Fe₃O₄, FeO, and/or Fe₂O₃.

7. The method of claim 1, wherein step (b) comprises electrolyzing the sample at a potential of from about 0.0 V to about 2.0 V.

8. The method of claim 1, wherein step (b) comprises electrolyzing the sample at a potential of from about 0.2 V to about 1.0 V.

9. The method of claim 1, wherein step (a) comprises adding hydrogen peroxide to the sample in an amount sufficient to make the sample about 0.1 M to about 10 mM H₂O₂.

10. The method of claim 1, wherein step (a) comprises adding hydrogen peroxide to the sample in an amount sufficient to make the sample about 0.1 to about 5 mM H₂O₂.

11. The method of claim 1, wherein step (a) comprises adding hydrogen peroxide to the sample in an amount sufficient to make the sample about 0.1 to about 1 mM H₂O₂.

12. A method to measure concentration of beta-lactoglobulin (BLG), the method comprising:

(a) adding hydrogen peroxide to a sample known to, or suspected of containing BLG;

(b) electrolyzing the sample of step (a) using a working electrode at a potential sufficient to electrolyze BLG, and measuring a current signal within the sample, wherein a diminution of the current signal in the sample as compared to a corresponding current signal from a standard solution containing hydrogen peroxide and no BLG indicates that the sample contains BLG; and

(c) determining the concentration of BLG in the sample by comparing the diminution of the current signal in step (b) to a standard curve of current signals compiled using solutions of known BLG concentration.

13. The method of claim 12, wherein step (b) comprises electrolyzing the sample using a working electrode comprising a transition metal or an oxide of a transition metal.

14. The method of claim 12, wherein step (b) comprises electrolyzing the sample using a working electrode comprising a transition metal.

15. The method of claim 12, wherein step (b) comprises electrolyzing the sample using a working electrode comprising an oxide of a transition metal.

16. The method of claim 12, wherein step (b) comprises electrolyzing the sample using a working electrode comprising an element selected from the group consisting of ruthenium, rhodium, palladium, platinum, silver, osmium, iridium, gold, mercury, rhenium, titanium, niobium, tantalum, or any combination thereof.

17. The method of claim 12, wherein step (b) comprises electrolyzing the sample using a working electrode comprising a metal oxide selected from the group consisting of Fe₃O₄, FeO, and/or Fe₂O₃.

18. The method of claim 12, wherein step (b) comprises electrolyzing the sample at a potential of from about 0.0 V to about 2.0 V.

19. The method of claim 12, wherein step (b) comprises electrolyzing the sample at a potential of from about 0.2 V to about 1.0 V.

20. The method of claim 12, wherein step (a) comprises adding hydrogen peroxide to the sample in an amount sufficient to make the sample about 0.1 M to about 10 mM H₂O₂.

21. The method of claim 12, wherein step (a) comprises adding hydrogen peroxide to the sample in an amount sufficient to make the sample about 0.1 to about 5 mM H₂O₂.

22. The method of claim 12, wherein step (a) comprises adding hydrogen peroxide to the sample in an amount sufficient to make the sample about 0.1 to about 1 mM H₂O₂.