Method and device for analysis and reading

• US 9,097,665 B2
• Filed: 08/26/2011
• Issued: 08/04/2015
• Est. Priority Date: 08/05/2011
• Status: Active Grant

• Current Assignee: Guangzhou Wondfo Biotech. Co., Ltd.
• Original Assignee: Guangzhou Wondfo Biotech. Co., Ltd.
• Inventors: Wang, Jihua, Wang, Zhicai
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Fisher, Brittany
• Application Number:
  US13/702,468
• Publication Number:
  US 20130183199A1
• Time in Patent Office: 1,439 Days
• Field of Search: 422/82.05
• US Class Current: 1/1
• CPC Class Codes: G01N 21/55 Specular reflectivity
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387

Abstract

An analyzing and reading device and an analyzing and reading method, for reading and analyzing a test strip for assay detection are disclosed. The test strip has a detection zone and a blank zone, the device includes a photoelectric detection circuit and a processor, wherein the photoelectric detection circuit includes at least two light sources which locate corresponding to the positions of the detection zone and the blank zone of the test strip and are able to emit lights corresponding to the detection zone and blank zone of the test strip, and at least one optical detectors which receive reflected lights from the above two zones; wherein lights emitted by the at least two light sources irradiate the detection zone and blank zone of the strip and are reflected therefrom, and then are received by the optical detector, which in turn feedback the detection information to the processor; the processor making analysis and decision based on the detection information received. The analyzing and reading device and method according to the present invention have high accuracy and less interference when reading.

Claims

1. An analyzing and reading device, for reading and analyzing a test strip for assay detection, the test trip having a detection zone and a blank zone, comprising:

a processor;

a photoelectric detection circuit, configured to detect signals of the strength of light reflection in the detection zone and the blank zone and feedback detection information to the processor;

wherein the photoelectric detection circuit comprises;

at least two light sources including a first light source and a second light source, corresponding to positions of the detection zone and the blank zone of the test strip, and able to emit lights corresponding to the detection zone and blank zone of the test strip; and

at least one optical detector, configured to receive reflected lights from the detection zone and the blank zone;

wherein the lights emitted by the at least two light sources irradiate the detection zone and blank zone of the strip and are reflected therefrom, and then received by the optical detector, which in turn feedback the detection information to the processor;

a diaphragm separating the first light source from the second light source and the at least one optical detector from the at least two light sources; and

a gap formed between the diaphragm and the test strip.

2. The analyzing and reading device according to claim 1, wherein the optical detector is a photoelectric sensor.

3. The analyzing and reading device according to claim 2, wherein the lights emitted by the two light sources irradiate the detection zone and blank zone of the test strip, and then are received by the photoelectric sensor after reflection.

4. The analyzing and reading device according to claim 3, wherein both the first and second light sources are light emitting diodes.

5. The analyzing and reading device according to claim 4, wherein both the first and second light sources are green light emitting diodes.

6. The analyzing and reading device according to claim 5, wherein wavelengths of the first and second light sources are the same.

7. The analyzing and reading device according to claim 1, wherein the processor is configured to emit light from the at least two light sources at different times.

8. The analyzing and reading device according to claim 3, wherein the diaphragm is a T-shaped diaphragm.

9. The analyzing and reading device according to claim 8, wherein the gap is formed between the T-shaped diaphragm among the first light source, the second light source and the photoelectric sensor, and the detection zone and blank zone of the test strip;

the lights emitted by the first and second light sources irradiate the detection and blank zones of the test strip, and then the reflected lights enter the photoelectric sensor through the gap and are received by the sensor.

10. The analyzing and reading device according to claim 1, wherein the device further comprises a housing made of light-tight synthetic plastic.

11. The analyzing and reading device according to claim 1, wherein the test strip comprises a porous water permeable carrier, which comprises a specific conjugation reagent with marker and a specific conjugation reagent without marker.

12. The analyzing and reading device according to claim 11, wherein the marker is colored particles.

13. An analyzing and reading method by using the analyzing and reading device according to claim 1, comprising the following steps:

controlling by the processor the respective lightening of the at least two light sources according to a timing design to irradiate the detection zone and the blank zone of the test strip;

receiving by the optical detector lights reflected;

then feeding back by the optical detector detection information to the processor; and

, making analysis and determination by the processor according to the detection information received.

14. The analyzing and reading device according to claim 1, wherein the processor is configured to:

receive the detection information from the optical detector;

determine an accumulation amount of a marker based on the detection information;

calculate a discriminant value based on the accumulation amount of the marker; and

compare the discriminant value with a threshold.