Imaging apparatus and image generating method
First Claim
Patent Images
1. An imaging apparatus, comprising:
- an imaging unit configured to capture a plurality of images of a fluorescent sample;
a pixel shift unit configured to change a relative position of the imaging unit for each of the plurality of images;
a processor; and
a memory storing computer readable instructions that, when executed by the processor, implement;
an image correction unit correcting, by setting as a reference image one of the plurality of images acquired by the imaging unit at one of the different relative positions, a gray-scale level of at least one correction target image selected from the plurality of images other than the reference image so that each histogram of each respective correction target image other than the reference image matches a histogram of the reference image; and
an image combination unit combining the reference image and each correction target image corrected by the image correction unit so as to generate image data with a higher resolution than each of the plurality of images.
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Abstract
An imaging apparatus includes: an imaging unit for capturing an image of a fluorescent sample; a pixel shift unit for changing the relative position of the imaging unit for the image; an image correction unit for correcting the gray-scale levels of plural pieces of image data using the histograms of the image data acquired by the imaging unit at different relative positions; and an image combination unit for combining the plural pieces of image data corrected by the image correction unit.
15 Citations
15 Claims
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1. An imaging apparatus, comprising:
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an imaging unit configured to capture a plurality of images of a fluorescent sample; a pixel shift unit configured to change a relative position of the imaging unit for each of the plurality of images; a processor; and a memory storing computer readable instructions that, when executed by the processor, implement; an image correction unit correcting, by setting as a reference image one of the plurality of images acquired by the imaging unit at one of the different relative positions, a gray-scale level of at least one correction target image selected from the plurality of images other than the reference image so that each histogram of each respective correction target image other than the reference image matches a histogram of the reference image; and an image combination unit combining the reference image and each correction target image corrected by the image correction unit so as to generate image data with a higher resolution than each of the plurality of images. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 10, 11)
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9. An image generating method, comprising:
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changing a relative position of an imaging unit for a plurality of images of a fluorescent sample and capturing each of the plurality of images of the fluorescent sample at a different relative position; setting, as a reference image, one of the plurality of images acquired at one of the different relative positions and correcting a gray-scale level of of at least one correction target image selected from the plurality of images other than the reference image so that each histogram of each respective correction target image other than the reference image matches a histogram of the reference image; and combining the reference image and each correction target image whose gray-scale levels have been corrected, so as to generate image data with a higher resolution than each of the plurality of images. - View Dependent Claims (12, 13, 14, 15)
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Specification