High-quality lipids produced by enzymatic liberation from biomass

• US 9,101,151 B2
• Filed: 05/05/2003
• Issued: 08/11/2015
• Est. Priority Date: 05/03/2002
• Status: Active Grant

• Current Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Original Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Inventors: Kobzeff, Joseph M., Weaver, Craig A.
• Primary Examiner(s): Ware, Debbie K
• Application Number:
  US10/513,576
• Publication Number:
  US 20060099693A1
• Time in Patent Office: 4,481 Days
• US Class Current: 1/1
• CPC Class Codes: A23D 9/00 Other edible oils or fats, e.g. shortenings, cooking oils
• CPC Class Codes: A23L 33/115 Fatty acids or derivatives thereof; Fats or oils
• CPC Class Codes: A23L 33/12 Fatty acids or derivatives thereof
• CPC Class Codes: A23L 33/40 Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A23V 2250/1882 Polyunsaturated fatty acids
• CPC Class Codes: A61K 31/202 having three or more double bonds, e.g. linolenic eicosanoids, e.g. leukotrienes A61K31/557
• CPC Class Codes: C11B 1/025 by enzymes or microorganisms, living or dead
• CPC Class Codes: C11B 3/001 by a combination of two or more of the means hereafter
• CPC Class Codes: C11B 5/0021 containing oxygen
• CPC Class Codes: C12N 1/06 Lysis of microorganisms
• CPC Class Codes: C12N 1/066 by physical methods
• CPC Class Codes: C12P 7/6472 containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Abstract

A high-quality lipid composition is disclosed having low oxidative deterioration such as measured by low anisidine values. Also disclosed are methods of preparing the same from a lipid-containing material that include enzymatic degradation of protein and/or carbohydrate components of the material. Lipid-containing materials include biomass, such as microorganisms. The invention further includes products containing the lipid compositions, such as dietary supplements, food products, pharmaceutical formulations, humanized animal milk, and infant formula.

Claims

1. A refined, bleached, or deodorized microbial or plant lipid comprising polyunsaturated fatty acid, wherein said lipid has an anisidine value of 1.5 or less, wherein said lipid has been liberated from biomass enzymatically and through addition of a surfactant, and wherein said polyunsaturated fatty acid present in said lipid comprises at least 20 weight percent docosahexaenoic acid, at least 5 weight percent docosapentaenoic acid, or at least 20 weight percent arachidonic acid.

2. The lipid of claim 1, wherein said polyunsaturated fatty acid is docosahexaenoic acid.

3. The lipid of claim 1, wherein said polyunsaturated fatty acid is docosapentaenoic acid.

4. The lipid of claim 1, wherein said polyunsaturated fatty acid is arachidonic acid.

5. The lipid of claim 1, wherein said polyunsaturated fatty acid comprises at least 30 weight percent docosahexaenoic acid.

6. The lipid of claim 1, wherein said polyunsaturated fatty acid comprises at least 35 weight percent docosahexaenoic acid.

7. The lipid of claim 1, wherein said polyunsaturated fatty acid comprises at least 10 weight percent docosapentaenoic acid.

8. The lipid of claim 1, wherein said polyunsaturated fatty acid comprises at least 15 weight percent docosapentaenoic acid.

9. The lipid of claim 1, wherein said polyunsaturated fatty acid comprises at least 20 weight percent docosapentaenoic acid.

10. The lipid of claim 1, wherein said polyunsaturated fatty acid comprises at least 30 weight percent arachidonic acid.

11. The lipid of claim 1, wherein said polyunsaturated fatty acid comprises at least 40 weight percent arachidonic acid.

12. The lipid of claim 1, wherein said polyunsaturated fatty acid comprises at least 50 weight percent arachidonic acid.

13. The lipid of claim 1, wherein said lipid is obtained from at least one of the group consisting of algae, bacteria, fungi and protists.

14. The lipid of claim 1, wherein said lipid is obtained from algae.

15. The lipid of claim 1, wherein said lipid is obtained from microorganisms selected from the group consisting of golden algae, green algae, dinoflagellates, yeast, fungi of the genus Mortierella, and Stramenopiles.

16. The lipid of claim 1, wherein said lipid is obtained from microorganisms selected from the group consisting of the genus Mortierella, genus Crypthecodinium, and order Thraustochytriales.

17. The lipid of claim 1, wherein said lipid is obtained from microorganisms selected from the group consisting of the genus Thraustochytrium, genus Schizochytrium or mixtures thereof.

18. The lipid of claim 1, wherein said lipid comprises a monoacylglyceride.

19. The lipid of claim 1, wherein said lipid comprises a diacylglyceride.

20. The lipid of claim 1, wherein said lipid comprises a triacylglyceride.

21. A product selected from the group consisting of dietary supplement, food product, pharmaceutical formulation, humanized animal milk, and infant formula, wherein the product comprises the lipid of claim 1.

22. A method of making a product selected from the group consisting of a dietary supplement, food product, pharmaceutical formulation, humanized animal milk, and infant formula, comprising adding the lipid of claim 1 to the dietary supplement, food product, pharmaceutical formulation, humanized animal milk, or infant formula.